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Literature summary extracted from

  • Akita, K.; Hieda, N.; Baba, N.; Kawaguchi, S.; Sakamoto, H.; Nakanishi, Y.; Yamanishi, M.; Mori, K.; Toraya, T.
    Purification and some properties of wild-type and N-terminal-truncated ethanolamine ammonia-lyase of Escherichia coli (2010), J. Biochem., 147, 83-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.7 expression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.7 additional information N-terminal truncated enzyme, similar to wild-type enzyme in catalytic properties, more resistant to p-chloromercuribenzoate than wild-type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.7 iodoacetamide
-
Escherichia coli
4.3.1.7 p-chlormercuribenzoate
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.7 additional information
-
adenosylcobalamin truncated enzymes, value between 0.000041 and 0.000078 Escherichia coli
4.3.1.7 additional information
-
ethanolamine truncated enzymes, value between 0.0059 and 0.0076 Escherichia coli
4.3.1.7 0.000055
-
adenosylcobalamin wild-type enzyme Escherichia coli
4.3.1.7 0.0082
-
ethanolamine wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.7 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.7 31% yield after DEAE-cellulose column Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.7 additional information
-
truncated enzymes, after purificaton, value between 12.45 and 63.1 Escherichia coli
4.3.1.7 55.1
-
wild-type enzyme, after purification Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.7 ethanolamine assay at pH 8.0, 37°C, reaction terminated by adding potassium citrate buffer Escherichia coli acetaldehyde + NH3
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.7 heterodecamer alphabeta, 6*49000, 6*32000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.3.1.7 ethanolamine ammonia-lyase
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.7 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.7 8
-
assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.7 adenosylcobalamin
-
Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.1.7 additional information
-
truncated enzymes, value around 0.0013 Escherichia coli p-chlormercuribenzoate
4.3.1.7 additional information
-
truncated enzymes, value between 0.07 to 0.24 Escherichia coli iodoacetamide
4.3.1.7 0.0003
-
wild-type enzyme Escherichia coli p-chlormercuribenzoate
4.3.1.7 0.07
-
wild-type enzyme Escherichia coli iodoacetamide