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Literature summary extracted from

  • Mediavilla, D.; Meton, I.; Baanante, I.V.
    Purification and kinetic characterization of 6-phosphofructo-1-kinase from the liver of gilthead sea bream (Sparus aurata) (2008), J. Biochem., 144, 235-244.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 D-fructose 2,6-bisphosphate converts saturation curves for D-fructose 6-phosphate to hyperbolic and activates PFK synergistically with AMP Sparus aurata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 3-phosphoglycerate about 70% inhibition at 7 mM Sparus aurata
2.7.1.11 ADP
-
 Sparus aurata
2.7.1.11 ATP complete inhibition at 10 mM Sparus aurata
2.7.1.11 citrate almost complete inhibition at 1 mM citrate in the absence of D-fructose 2,6-bisphosphate Sparus aurata
2.7.1.11 additional information not inhibited by 6-phosphogluconate and phosphocreatine Sparus aurata
2.7.1.11 phosphoenolpyruvate about 30% inhibition at 5 mM Sparus aurata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.051
-
 ATP purified enzyme, at pH 7.0, in the absence of D-fructose 2,6-bisphosphate Sparus aurata
2.7.1.11 0.076
-
 ATP purified enzyme, at pH 7.0, in the presence of 0.005 mM D-fructose 2,6-bisphosphate Sparus aurata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 86000
-
 6 * 86000, apparent molecular weight estimated from SDS-PAGE Sparus aurata
2.7.1.11 510000
-
 native enzyme, gel filtration Sparus aurata

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Sparus aurata
-
 gilthead sea bream
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 12% PEG6000 precipitation, ATP agarose column chromatography, DEAE-Sepharose column chromatography, phosphocellulose column chromatography, and Blue-Sepharose column chromatography Sparus aurata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.11 liver
-
 Sparus aurata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 0.04
-
 crude extract Sparus aurata
2.7.1.11 627
-
 after 15700fold purification Sparus aurata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Sparus aurata ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 homohexamer 6 * 86000, apparent molecular weight estimated from SDS-PAGE Sparus aurata

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 6-phosphofructo-1-kinase
-
 Sparus aurata
2.7.1.11 PFK
-
 Sparus aurata

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.11 ATP
-
 Sparus aurata