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Literature summary extracted from

  • Sun, Y.H.; Cheng, Q.; Tian, W.X.; Wu, X.D.
    A substitutive substrate for measurements of beta-ketoacyl reductases in two fatty acid synthase systems (2008), J. Biochem. Biophys. Methods, 70, 850-856.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 NH2-terminally His-tagged FabG expressed in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.100 epigallocatechin gallate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.01
-
 NADPH
-
 Escherichia coli
1.1.1.100 0.127
-
 ethyl acetoacetate
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100 by nickel affinity chromatography Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.100 -20°C, phosphate buffer, 10% glycerol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 ethyl acetoacetate + NADPH
-
 Escherichia coli ethyl 3-hydroxybutyrate + NADP+
-
 ?
1.1.1.100 methyl acetoacetate + NADPH is not a good substrate as ethyl acetoacetate Escherichia coli ? + NADP+
-
 ?
1.1.1.100 propyl acetoacetate + NADPH as substrate it is limited to its weak solubility Escherichia coli ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 beta-ketoacyl-ACP reductase
-
 Escherichia coli
1.1.1.100 FabG
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.100 45
-
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.100 0.3
-
 ethyl acetoacetate
-
 Escherichia coli
1.1.1.100 0.59
-
 NADPH
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.100 8
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.100 0.00554
-
 at pH 8.0 Escherichia coli epigallocatechin gallate
1.1.1.100 0.0084
-
 at pH 8.3 Escherichia coli epigallocatechin gallate
1.1.1.100 0.0105
-
 at pH 7.5 Escherichia coli epigallocatechin gallate
1.1.1.100 0.0336
-
 at pH 8.5 Escherichia coli epigallocatechin gallate
1.1.1.100 0.0578
-
 at pH 7.0 Escherichia coli epigallocatechin gallate
1.1.1.100 0.12
-
 at pH 9.0 Escherichia coli epigallocatechin gallate
1.1.1.100 0.1223
-
 at pH 6.5 Escherichia coli epigallocatechin gallate