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Literature summary extracted from

  • Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
    Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence (1998), J. Bacteriol., 180, 1607-1617.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.B9 synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris
2.4.1.251 synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris
2.4.1.252 synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris
2.4.1.264 synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris
2.7.8.31 synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.31 the C-terminal portion of GumD is cloned into broad-host-range vector pRK404, producing plasmid pCD2. The C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B9 additional information Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan ?
-
 ?
2.4.1.251 GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol Xanthomonas campestris
-
 GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
 ?
2.4.1.264 UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol
-
 ?
2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate Xanthomonas campestris the enzyme is involved in biosynthesis of xanthan UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B9 Xanthomonas campestris
-
 pv. campestris
-
2.4.1.251 Xanthomonas campestris
-
 pv. campestris
-
2.4.1.252 Xanthomonas campestris
-
 pv. campestris
-
2.4.1.264 Xanthomonas campestris
-
 pv. campestris
-
2.5.1.95 Xanthomonas campestris pv. campestris
-
-
-
2.7.8.31 Xanthomonas campestris
-
 pv. campestris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B9 additional information the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris ?
-
 ?
2.4.1.B9 UDP-glucose + D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 Xanthomonas campestris UDP + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
2.4.1.251 GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 Xanthomonas campestris GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
-
 Xanthomonas campestris GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
 ?
2.4.1.252 GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
 ?
2.4.1.264 UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol
-
 ?
2.4.1.264 UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol
-
 Xanthomonas campestris UDP + GlcA-beta-(1-2)-D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol
-
 ?
2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.31 UDP-glucose + ditrans,octacis-undecaprenyl phosphate the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity Xanthomonas campestris UMP + alpha-D-glucopyranosyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B9 gumH
-
 Xanthomonas campestris
2.4.1.251 gumI
-
 Xanthomonas campestris
2.4.1.252 gumH
-
 Xanthomonas campestris
2.4.1.264 gumK
-
 Xanthomonas campestris
2.5.1.95 GumL
-
 Xanthomonas campestris pv. campestris

General Information

EC Number General Information Comment Organism
2.4.1.B9 malfunction no mutation of the gumM gene without further chromosomal rearrangements. Inactivation of gumM completely abolishes in vitro polymer formation Xanthomonas campestris
2.4.1.B9 physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris
2.4.1.251 malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumK completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Accumulation of GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha1-diphospho-(lipid carrier) in the gumI deletion strain. Mutations in gumI have less effect on the amount of in vitro produced polysaccharide. The gumI deletion strain produces about 10% of the polymer amount produced by the wild-type strain Xanthomonas campestris
2.4.1.251 physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris
2.4.1.252 malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumH completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Oligosaccharides obtained from gumH- strain XcH can be resolved into two components: a compound with the same mobility as the disaccharide cellobiose and glucose. XcH cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose do not incorporate radioactivity into the 1203 extract Xanthomonas campestris
2.4.1.252 physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris
2.4.1.264 malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumK completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDP-mannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. The gumK deletion strain produces a very low amount of polymer in vivo (polytrimer). Mutations in gumK gene has less effect on the amount of in vitro produced polysaccharide Xanthomonas campestris
2.4.1.264 physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris
2.5.1.95 malfunction the mutant strain is unable to synthesize the pyruvylated intermediate in vitro. Enzyme activity is not essential for polysaccharide production since inactivation of gumL does not affect polymerization. gumL mutants are not affected in growth and produce normal nonpyruvylated polymer levels Xanthomonas campestris pv. campestris
2.7.8.31 malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumD completely abolishes in vitro polymer formation. The gumD- mutant is the unique mutant strain, generated in a wild-type background, that shows no released labeled oligosaccharides when permeabilized cells are labeled with UDP-[14C]glucose. Cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose show similar results. The C-terminal portion of GumD is cloned into broad-host-range vector pRK404, producing plasmid pCD2. This plasmid complements the xanthan defect in the XcD strain, rendering mucoid colonies Xanthomonas campestris
2.7.8.31 physiological function the enzyme is involved in biosynthesis of the exopolysaccharide xanthan Xanthomonas campestris