Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Zhang, Y.H.
    A highly active phosphoglucomutase from Clostridium thermocellum: cloning, purification, characterization and enhanced thermostability (2010), J. Appl. Microbiol., 108, 39-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

General Stability

EC Number General Stability Organism
5.4.2.2 at pH 7.5, the PGM activity in the HEPES buffer is 20% lower than in the Tris-HCl buffer Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.41
-
 alpha-D-glucose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus
5.4.2.2 0.44
-
 alpha-D-mannose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+ activator Acetivibrio thermocellus
5.4.2.2 Mn2+ activator Acetivibrio thermocellus
5.4.2.2 additional information Zn2+, Cu2+ and Pd2+ do not enhance the PGM activity Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 64900
-
 estimated from amino acid sequence Acetivibrio thermocellus
5.4.2.2 65000
-
 gel filtration Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 ultracentrifugation Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus alpha-D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-mannose 1-phosphate
-
 Acetivibrio thermocellus alpha-D-mannose 6-phosphate
-
 ?
5.4.2.2 additional information PGM from Clostridium thermocellum is a dual-specificity enzyme with phosphoglucomutase and phosphomannomutase activities Acetivibrio thermocellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 monomer 1 * 65000, gel filtration Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 PGM
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.2 70
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.2.2 50 80
-
 Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.2 60 70 at 60°C PGM is relatively stable and loses 10% of its initial activity after incubation for 8 h. At 70°C, it deactivates quickly with a half-life of 0.8 h. PGM at a concentration of 500 nM has a half-life of 30 h at 60°C, while 2 nM or 50 nM PGM loses nearly 100% of activity within 12 h. Thermostability of PGM at a low concentration (2 nM, 100 units/l) is enhanced by 12fold at 60°C with addition of 1 mg/ml bovine serum albumin, 0.1% (v/v) Triton X-100, 5 mM Mg2+, and 0.5 mM Mn2+ Acetivibrio thermocellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.2 59
-
 alpha-D-mannose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus
5.4.2.2 190
-
 alpha-D-glucose 1-phosphate recombinant wild type enzyme, at 60°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.2 8.8
-
 around pH 8.8 Acetivibrio thermocellus