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Literature summary extracted from

  • Melzer, M.; Chen, J.C.; Heidenreich, A.; Gaeb, J.; Koller, M.; Kehe, K.; Blum, M.M.
    Reversed enantioselectivity of diisopropyl fluorophosphatase against organophosphorus nerve agents by rational design (2009), J. Am. Chem. Soc., 131, 17226-17232.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.2 expressed in Escherichia coli BL21 cells Loligo vulgaris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.8.2 mutant enzymes are crystallized at room temperature by hanging drop vapor diffusion method, using 0.1 M Tris buffer pH 8.5, 2% tacsimate, 16% (w/v) PEG 3350 for mutant E37A/Y144A/R146A/T195M or 0.2 M KCl, 0.05 M HEPES buffer pH 7.5, 35% (w/v) pentaerythriol propoxylate for mutant E37D/Y144A/R16A/T195M Loligo vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
3.1.8.2 E37A/Y144A/R146A/T195M the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme Loligo vulgaris
3.1.8.2 E37D/Y144A/R16A/T195M the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme Loligo vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.8.2 2.12
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 3.76
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
3.1.8.2 3.93
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.2 Loligo vulgaris Q7SIG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.2
-
 Loligo vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.2 (R)-cyclosarin + H2O
-
 Loligo vulgaris ?
-
 ?
3.1.8.2 (R)-sarin + H2O
-
 Loligo vulgaris ?
-
 ?
3.1.8.2 (S)-cyclosarin + H2O
-
 Loligo vulgaris ?
-
 ?
3.1.8.2 (S)-sarin + H2O
-
 Loligo vulgaris ?
-
 ?
3.1.8.2 diisopropyl fluorophosphate + H2O
-
 Loligo vulgaris diisopropyl phosphate + fluoride
-
 ?
3.1.8.2 soman + H2O
-
 Loligo vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.2 DFPase
-
 Loligo vulgaris
3.1.8.2 diisopropyl fluorophosphatase
-
 Loligo vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.8.2 211
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
3.1.8.2 291
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 376
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.8.2 17
-
 (S)-cyclosarin wild type enzyme Loligo vulgaris
3.1.8.2 24
-
 (R)-sarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
3.1.8.2 24
-
 (S)-cyclosarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
3.1.8.2 27
-
 (R)-cyclosarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
3.1.8.2 42
-
 (S)-sarin wild type enzyme Loligo vulgaris
3.1.8.2 45
-
 (R)-sarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 47
-
 (R)-sarin wild type enzyme Loligo vulgaris
3.1.8.2 56
-
 diisopropyl fluorophosphate wild type enzyme Loligo vulgaris
3.1.8.2 71
-
 (S)-sarin mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
3.1.8.2 96
-
 diisopropyl fluorophosphate mutant enzyme E37D/Y144A/R16A/T195M Loligo vulgaris
3.1.8.2 130
-
 (R)-cyclosarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 140
-
 diisopropyl fluorophosphate mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 230
-
 (S)-sarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 490
-
 (S)-cyclosarin mutant enzyme E37A/Y144A/R146A/T195M Loligo vulgaris
3.1.8.2 720
-
 (R)-cyclosarin wild type enzyme Loligo vulgaris