Literature summary extracted from
Liptak, M.D.; Datta, S.; Matthews, R.G.; Brunold, T.C.
Spectroscopic study of the cobalamin-dependent methionine synthase in the activation conformation: effects of the Y1139 residue and S-adenosylmethionine on the B12 cofactor (2008), J. Am. Chem. Soc., 130, 16374-16381.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.1.1.13 |
mutant enzymes I690C/G743C and I690C/G743C/Y1139 are expressed in Escherichia coli Hms174(DE3) cells |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.1.1.13 |
I690C/G743C |
the variant of a C-terminal fragment of MetH locks the enzyme into the activation conformation without perturbing any of the residues in the vicinity of the active site |
Escherichia coli |
2.1.1.13 |
I690C/G743C/Y1139F |
the I690C/G743C MetH variant is trapped in the activation conformation |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.1.1.13 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.1.1.13 |
Hi-Trap column chromatography and MonoQ column chromatography |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.1.1.13 |
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine |
- |
Escherichia coli |
tetrahydropteroylmonoglutamate + L-methionine |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.1.1.13 |
MetH |
- |
Escherichia coli |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.1.1.13 |
vitamin B12 |
dependent on |
Escherichia coli |
|