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Literature summary extracted from
- Effect of BTH on anthocyanin content and activities of related enzymes in Strawberry after harvest (2010), J. Agric. Food Chem., 58, 5801-5805.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.25 | benzo-thiadiazole-7-carbothioic acid S-methyl ester | activates the enzymes of the anthocyanin metabolism, including the shikimate dehydrogenase, and increases the anthocyanin content in strawberries after harvest, with SKDH playing a crucial role | Fragaria x ananassa |  |
| 1.1.1.25 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 1.1.1.49 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 1.1.1.219 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 1.14.14.91 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 4.3.1.23 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 4.3.1.24 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
| 6.2.1.12 | S-methyl 1,2,3-benzothiadiazole-7-carbothioate | treatment of fruits | Fragaria x ananassa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | Fragaria x ananassa | - |
3-dehydroshikimate + NADPH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.25 | Fragaria x ananassa | - |
- |
- |
| 1.1.1.49 | Fragaria x ananassa | - |
- |
- |
| 1.1.1.219 | Fragaria x ananassa | - |
- |
- |
| 1.14.14.91 | Fragaria x ananassa | - |
- |
- |
| 4.3.1.23 | Fragaria x ananassa | - |
- |
- |
| 4.3.1.24 | Fragaria x ananassa | - |
- |
- |
| 6.2.1.12 | Fragaria x ananassa | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.25 | fruit | after harvest, storage at 1°C, SKDH activity increases in all strawberries | Fragaria x ananassa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | additional information | - |
during storage at 1°C, SKDH activity increases in all strawberries | Fragaria x ananassa |
| 1.1.1.25 | 95 | - |
value about, fruits without any treatment | Fragaria x ananassa |
| 1.1.1.25 | 184 | - |
after treatment with benzo-thiadiaziole-7-carbothioic acid S-methyl ester | Fragaria x ananassa |
| 1.1.1.25 | 184 | - |
pH 9.0, 25°C, fruits after harvest | Fragaria x ananassa |
| 1.1.1.49 | 22.2 | - |
untreated fruits | Fragaria x ananassa |
| 1.1.1.49 | 36.3 | - |
fruits treated with benzo-thiadiaziole-7-carbothioic acid S-methyl ester | Fragaria x ananassa |
| 1.1.1.219 | 156.4 | - |
without any fruit treatment, day 4 of storage at 1°C | Fragaria x ananassa |
| 1.14.14.91 | additional information | - |
55 U/mg protein, value about, 1 unit is defined as the amount of enzyme causing an increase in absorption, without any treatment, storage at 1°C for 10 days | Fragaria x ananassa |
| 1.14.14.91 | additional information | - |
70 U/mg protein, value about, 1 unit is defined as the amount of enzyme causing an increase in absorption, after treatment of fruits with benzo-thiadiaziole-7-carbothioic acid S-methyl ester, storage at 1°C for 10 days | Fragaria x ananassa |
| 4.3.1.23 | additional information | - |
80.3 U/mg protein, 1 unit is defined as the amount of enzyme causing an increase in absorbtion | Fragaria x ananassa |
| 4.3.1.24 | additional information | - |
44.5 U/mg protein, 1 unit is defined as the amount of enzyme causing an increase in absorbtion | Fragaria x ananassa |
| 6.2.1.12 | additional information | - |
38.3 U/mg protein, 1 unit is defined as the amount of enzyme causing an increase in absorbtion, day 2 of storage at 1°C | Fragaria x ananassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.25 | 1°C, storage for 10 days, increase of enzyme activity | Fragaria x ananassa |
| 1.1.1.25 | storage at 1°C, SKDH activity increases in all strawberries | Fragaria x ananassa |
| 1.1.1.219 | 1°C, after 4 days of storage decrease in enzyme activity | Fragaria x ananassa |
| 1.14.14.91 | 1°C, during first 8 days decrease of enzyme activity, with prolonged stroage time increase of enzyme activity | Fragaria x ananassa |
| 6.2.1.12 | 1°C, after two days decrease in enzyme activity | Fragaria x ananassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.25 | shikimate + NADP+ | - |
Fragaria x ananassa | 3-dehydroshikimate + NADPH + H+ | - |
r | |
| 1.1.1.25 | shikimate + NADP+ | assay at 24°C, pH 9.0 | Fragaria x ananassa | 3-dehydroshikimate + NADPH | - |
? | |
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | assay at pH 7.4 | Fragaria x ananassa | NADPH + ? | - |
? | |
| 1.1.1.219 | dihydroquercetin + NADPH | assay at 25°C, pH 7.5 | Fragaria x ananassa | ? | - |
? | |
| 1.14.14.91 | trans-cinnamic acid + [reduced NADPH-hemoprotein reductase] + O2 | assay at 37°C, pH 7.5, reaction stopped with HCl | Fragaria x ananassa | ? | - |
? | |
| 4.3.1.23 | L-tyrosine | assay at 40°C, pH 7.8 | Fragaria x ananassa | trans-p-hydroxycinnamate + NH3 | - |
? | |
| 4.3.1.24 | L-phenylalanine | assay at 40°C, pH 7.8 | Fragaria x ananassa | (E)-cinnamate + NH3 | - |
? | |
| 6.2.1.12 | ATP + 4-coumarate + CoA | assay at pH 7.5 | Fragaria x ananassa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.25 | shikimate dehydrogenase | - |
Fragaria x ananassa |
| 1.1.1.25 | SKDH | - |
Fragaria x ananassa |
| 1.1.1.49 | G6PDH | - |
Fragaria x ananassa |
| 1.1.1.49 | glucose-6-phosphate dehydrogenase | - |
Fragaria x ananassa |
| 1.1.1.219 | DFR | - |
Fragaria x ananassa |
| 1.1.1.219 | dihydroflavonol 4-reductase | - |
Fragaria x ananassa |
| 1.14.14.91 | C4H | - |
Fragaria x ananassa |
| 1.14.14.91 | cinnamate-4-hydroxylase | - |
Fragaria x ananassa |
| 4.3.1.23 | TAL | - |
Fragaria x ananassa |
| 4.3.1.23 | tyrosine ammonia lyase | - |
Fragaria x ananassa |
| 4.3.1.24 | PAL | - |
Fragaria x ananassa |
| 4.3.1.24 | phenylalanine ammonia lyase | - |
Fragaria x ananassa |
| 6.2.1.12 | 4-CL | - |
Fragaria x ananassa |
| 6.2.1.12 | 4-coumarate/coenzyme A ligase | - |
Fragaria x ananassa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 24 | - |
assay at | Fragaria x ananassa |
| 1.1.1.25 | 25 | - |
assay at | Fragaria x ananassa |
| 1.1.1.219 | 25 | - |
assay at | Fragaria x ananassa |
| 1.14.14.91 | 37 | - |
assay at | Fragaria x ananassa |
| 4.3.1.23 | 40 | - |
assay at | Fragaria x ananassa |
| 4.3.1.24 | 40 | - |
assay at | Fragaria x ananassa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.25 | 9 | - |
assay at | Fragaria x ananassa |
| 1.1.1.49 | 7.4 | - |
assay at | Fragaria x ananassa |
| 1.1.1.219 | 7.5 | - |
assay at | Fragaria x ananassa |
| 1.14.14.91 | 7.5 | - |
assay at | Fragaria x ananassa |
| 4.3.1.23 | 7.8 | - |
assay at | Fragaria x ananassa |
| 4.3.1.24 | 7.8 | - |
assay at | Fragaria x ananassa |
| 6.2.1.12 | 7.5 | - |
assay at | Fragaria x ananassa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.25 | NADP+ | - |
Fragaria x ananassa | |
| 1.1.1.25 | NADPH | - |
Fragaria x ananassa | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.25 | metabolism | SKDH is one of the crucial enzymes in the biosynthesis of anthocyanin metabolites, overview | Fragaria x ananassa |
| 1.1.1.219 | metabolism | key regulatory enzyme of the flavonoid pathway | Fragaria x ananassa |
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