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Literature summary extracted from

  • Ko, Y.T.; Chung, P.S.; Shih, Y.C.; Chang, J.W.
    Cloning, characterization, and expression of mungbean (Vigna radiata L.) starch branching enzyme II cDNA in Escherichia coli (2009), J. Agric. Food Chem., 57, 871-879.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expression in Escherichia coli BL21 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 108000
-
 Western blot Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Vigna radiata Q4VUI1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 25.4fold after immobilized metal-ion affinity chromatography Vigna radiata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.18 additional information
-
 0.252 U/mg for the crude enzyme, 1 U = decrease of 1.0 units of absorbance per minute Vigna radiata
2.4.1.18 additional information
-
 6.402 U/mg for the purified enzyme, 1 U = decrease of 1.0 units of absorbance per minute Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylose assay at pH 7.0, 30°C for 90 min Vigna radiata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 full-length starch branching enzyme II
-
 Vigna radiata
2.4.1.18 SBE
-
 Vigna radiata
2.4.1.18 VsbeII
-
 Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 30
-
 assay at Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7
-
 assay at Vigna radiata