EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.9 | AACT, DNA and amino acid sequence determination and analysis, sequence comparison with Arabidopsis thaliana, expression of the active His-tagged enzyme in Escherichia coli TOP10 cells | Helianthus annuus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.9 | glyoxysome | - |
Helianthus annuus | 9514 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 47000 | - |
x * 47000, recombinant enzyme, SDS-PAGE | Helianthus annuus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.9 | Helianthus annuus | D2IH11 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.9 | recombinant His-tagged AACT 88fold to homogeneity by nickel affinity chromatography from Escherichia coli TOP10 cells | Helianthus annuus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.9 | cotyledon | - |
Helianthus annuus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 13.2 | - |
purified native enzyme | Helianthus annuus |
2.3.1.9 | 15.78 | - |
purified recombinant enzyme | Helianthus annuus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | acetoacetyl-CoA + CoA | coupled reaction assay in which the product of the thiolase reaction, acetyl-CoA, becomes the substrate for citrate synthase | Helianthus annuus | 2 acetyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.9 | ? | x * 47000, recombinant enzyme, SDS-PAGE | Helianthus annuus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.9 | AACT | - |
Helianthus annuus |
2.3.1.9 | acetoacetyl CoA thiolase | - |
Helianthus annuus |
2.3.1.9 | thiolase II | - |
Helianthus annuus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 8.5 | - |
assay at | Helianthus annuus |