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Literature summary extracted from

  • Panayiotou, C.; Solaroli, N.; Johansson, M.; Karlsson, A.
    Evidence of an intact N-terminal translocation sequence of human mitochondrial adenylate kinase 4 (2010), Int. J. Biochem. Cell Biol., 42, 62-69.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.3 K4G when the amino acid residue is mutated, the protein is imported in mitochondria Homo sapiens
2.7.4.3 K4G/R7G when both amino acid residues are mutated simultaneously the protein remains in the cytosol Homo sapiens
2.7.4.3 R7G when the amino acid residue is mutated, the protein is imported in mitochondria Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.0014
-
 AMP recombinant adenylate kinase 4, using GTP as cosubstrate Homo sapiens
2.7.4.3 0.0053
-
 AMP recombinant adenylate kinase 4, using ATP as cosubstrate Homo sapiens
2.7.4.3 0.507
-
 dAMP recombinant adenylate kinase 4, using ATP as cosubstrate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 53000
-
 SDS-PAGE, fused with the GST tag the recombinant protein has a size of about 53000 Da Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Homo sapiens P27144
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 glutathione-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP adenylate kinase 4 shows slightly lower efficiency for the phosphorylation of AMP with ATP compared to the phosphorylation of AMP with GTP Homo sapiens ADP + ADP
-
 ?
2.7.4.3 ATP + CMP phosphorylation of CMP is also detected but to a lesser extend Homo sapiens ADP + CDP
-
 ?
2.7.4.3 ATP + dAMP dAMP is the poorest substrate Homo sapiens ADP + dADP
-
 ?
2.7.4.3 GTP + AMP isozyme adenylate kinase 4 shows its highest efficiency when phosphorylating AMP with GTP, when GTP is used as phosphate donor only AMP is clearly phosphorylated and the phosphorylation efficiency for dAMP, CMP and dCMP is very low Homo sapiens GDP + ADP
-
 ?
2.7.4.3 additional information adenylate kinase 4 catalyzes the phosphorylation of AMP, dAMP,CMPand dCMP with ATP or GTP as phosphate donors and also phosphorylates AMP with UTP as phosphate donor Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.3 adenylate kinase 4
-
 Homo sapiens
2.7.4.3 AK4 isozyme Homo sapiens