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Literature summary extracted from

  • Tuckey, R.C.; Nguyen, M.N.; Slominski, A.
    Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cyclodextrin (2008), Int. J. Biochem. Cell Biol., 40, 2619-2626.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.6 additional information vitamin D3 and cholesterol are metabolized with comparable efficiencies in cyclodextrin, but the Km for both show a strong dependence on the cyclodextrin concentration, decreasing with decreasing cyclodextrin Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.0091
-
 cholesterol in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 0.0296
-
 vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 0.067
-
 20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 1.18
-
 cholesterol in the presence of 4.5% cyclodextrin Bos taurus
1.14.15.6 3.67
-
 vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 membrane
-
 Bos taurus 16020
-
1.14.15.6 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 20,23-dihydroxyvitamin D3 + reduced adrenodoxin + O2
-
 Bos taurus 17,20,23-trihydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 20-hydroxyvitamin D3 + reduced adrenodoxin + O2
-
 Bos taurus 20,23-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 vitamin D3 + reduced adrenodoxin + O2 is hydroxylated with lower efficiency than cholesterol Bos taurus 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.6 CYP11A1
-
 Bos taurus
1.14.15.6 cytochrome P450scc
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.6 0.1
-
 cholesterol in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 0.123
-
 20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 0.248
-
 cholesterol in the presence of 4.5% cyclodextrin Bos taurus
1.14.15.6 0.328
-
 vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 1.6
-
 vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.15.6 0.212
-
 cholesterol in the presence of 4.5% cyclodextrin Bos taurus
1.14.15.6 0.435
-
 vitamin D3 in the presence of 4.5% cyclodextrin Bos taurus
1.14.15.6 1.83
-
 20-hydroxyvitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 11.1
-
 vitamin D3 in the presence of 0.45% cyclodextrin Bos taurus
1.14.15.6 11.17
-
 cholesterol in the presence of 0.45% cyclodextrin Bos taurus