Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhan, B.; Perally, S.; Brophy, P.M.; Xue, J.; Goud, G.; Liu, S.; Deumic, V.; de Oliveira, L.M.; Bethony, J.; Bottazzi, M.E.; Jiang, D.; Gillespie, P.; Xiao, S.H.; Gupta, R.; Loukas, A.; Ranjit, N.; Lustigman, S.; Oksov, Y.; Hotez, P.
    Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus (2010), Infect. Immun., 78, 1552-1563.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.18 medicine the GST isozymes are useful as vaccine against hookworm infection Necator americanus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 genes Na-gst-1, Na-gst-2, and Na-gst-3, encoding isozymes GST-1, GST-2, and GST-3, phylogenetic analysis, recombinant expression in Pichia pastoris, the recombinant isozymes all exhibit low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities Necator americanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Hematin binding by isozymes with high affinity Necator americanus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Necator americanus D3U1A5 genes Na-gst-1, Na-gst-2, and Na-gst-3 encoding isozymes GST-1, GST-2, and GST-3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Necator americanus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 additional information recombinant isozymes GST-1, GST-2, and GST-3, expressed in Pichia pastoris, all exhibit low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities Necator americanus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Necator americanus
2.5.1.18 GST
-
 Necator americanus
2.5.1.18 More the Necator americanus GST isozymes belong to a nematode-specific nu-class GST family Necator americanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
 assay at Necator americanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5
-
 assay at Necator americanus

General Information

EC Number General Information Comment Organism
2.5.1.18 physiological function the isozymes may be involved in the detoxification and/or transport of heme Necator americanus