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Literature summary extracted from

  • Perrine, C.; Ju, T.; Cummings, R.D.; Gerken, T.A.
    Systematic determination of the peptide acceptor preferences for the human UDP-Gal:glycoprotein-alpha-GalNAc beta 3 galactosyltransferase (T-synthase) (2009), Glycobiology, 19, 321-328.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.122 CaCl2 0.1 mM are included in assay medium Homo sapiens
2.4.1.122 MnCl2 20 mM are included in assay medium Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.122 additional information Homo sapiens elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc ?
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine Homo sapiens
-
 UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.122 additional information
-
 T-synthase may utilize charge-charge interactions to modulate its activity, rejecting basic peptides over neutral and acidic peptide acceptors, T-synthase recognizes specific features of the peptide portion of the substrate, including overall charge and the presence of Gly, Phe, Tyr, and perhaps Pro at specific positions, up to four residues from the site of glycosylation, and indeed may use these features to modulate sites of Core 1 glycosylation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 additional information elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc Homo sapiens ?
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine
-
 Homo sapiens UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 T-synthase
-
 Homo sapiens
2.4.1.122 UDP-Gal:glycoprotein-alpha-GalNAc beta3 galactosyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.122 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.122 7.2
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.122 physiological function mucin-type protein O-glycosylation Homo sapiens