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Literature summary extracted from

  • Konishi, T.; Takeda, T.; Miyazaki, Y.; Ohnishi-Kameyama, M.; Hayashi, T.; O'Neill, M.A.; Ishii, T.
    A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose (2006), Glycobiology, 17, 345-354.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.30 expressed in Escherichia coli Oryza sativa

General Stability

EC Number General Stability Organism
5.4.99.30 repeated cycles of freezing and thawing did not significantly affect mutase activity Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.30 EDTA 5 mM, complete inhibition; 5 mM, complete inhibition Oryza sativa
5.4.99.30 additional information no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM; no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM Oryza sativa
5.4.99.30 UDP-Gal decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose Oryza sativa
5.4.99.30 UDP-Glc decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose Oryza sativa
5.4.99.30 UDP-Xyl decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose; decrease in mutase activity, when the nucleotide sugar reacts with the enzyme prior to the addition of beta-L-arabinofuranose Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.30 0.0531
-
 UDP-beta-L-arabinofuranose pH 6.8, 25°C Oryza sativa
5.4.99.30 0.055
-
 UDP-beta-L-arabinopyranose pH 6.8, 25°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.30 cytosol
-
 Oryza sativa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.30 Mn2+ 5 mM, activity almost doubles Oryza sativa
5.4.99.30 additional information no affect: CaCl2, MgCl2, CuSO4, ZnCl2, and CoCl2 at 5 mM Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.30 41000
-
 x * 41000, SDS-PAGE Oryza sativa
5.4.99.30 460000
-
 mutase is likely to exist as a complex composed of numerous proteins, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.30 UDP-beta-L-arabinofuranose Oryza sativa
-
 UDP-beta-L-arabinopyranose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.30 Oryza sativa O82706 fragment; L. cv. Koshihikari
-
5.4.99.30 Oryza sativa Q8H8T0 L. cv. Koshihikari
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.4.99.30 glycoprotein rUAM1 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc Oryza sativa
5.4.99.30 glycoprotein rUAM3 is autoglycosylated when each protein reacts separately with UDP-Glc. Reversibly glycosylated in the presence of UDP-Glc Oryza sativa

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.30
-
 Oryza sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.30 UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) Oryza sativa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.30 seedling
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.30 35.2
-
-
 Oryza sativa

Storage Stability

EC Number Storage Stability Organism
5.4.99.30 -20°C, mutase activity is stable for at least 3 months Oryza sativa
5.4.99.30 4°C, mutase activity is stable for 3 weeks Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.30 UDP-beta-L-arabinofuranose
-
 Oryza sativa UDP-beta-L-arabinopyranose
-
 r
5.4.99.30 UDP-beta-L-arabinofuranose the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) Oryza sativa UDP-beta-L-arabinopyranose
-
 r
5.4.99.30 UDP-beta-L-arabinofuranose the reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) Oryza sativa UDP-beta-L-arabinopyranose
-
 ?
5.4.99.30 UDP-beta-L-arabinopyranose
-
 Oryza sativa UDP-beta-L-arabinofuranose
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.99.30 ? x * 41000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
5.4.99.30 Os03g40270 protein
-
 Oryza sativa
5.4.99.30 Os07g41360 protein
-
 Oryza sativa
5.4.99.30 RGP1
-
 Oryza sativa
5.4.99.30 RGPs3
-
 Oryza sativa
5.4.99.30 UAM1
-
 Oryza sativa
5.4.99.30 UAMs3
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.30 55
-
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.30 5.5 6 formation of beta-L-arabinofuranose Oryza sativa
5.4.99.30 7 7.5 formation of beta-L-arabinopyranose Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.99.30 additional information the enzyme does not require a cofactor Oryza sativa