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Literature summary extracted from

  • Park, H.; Ahn, I.Y.; Lee, J.K.; Shin, S.C.; Lee, J.; Choy, E.J.
    Molecular cloning, characterization, and the response of manganese superoxide dismutase from the Antarctic bivalve Laternula elliptica to PCB exposure (2009), Fish Shellfish Immunol., 27, 522-528.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 MnSOD, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, and quantitative real-time RT-PCR expression analysis, expression in Escherichia coli strain BL21(DE3) Laternula elliptica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn2+ a MnSOD, activates, binding sequence is DVWEHAYY Laternula elliptica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24800
-
 x * 24800, about, sequence calculation Laternula elliptica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Laternula elliptica
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Laternula elliptica C7DRQ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant enzyme from Escherichia coli strain BL21(DE3) Laternula elliptica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 20.1
-
 purified recombinant enzyme, in absence of Mn2+ Laternula elliptica
1.15.1.1 1853
-
 purified recombinant enzyme, in presence of Mn2+ Laternula elliptica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+
-
 Laternula elliptica O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+ enzyme activity assay by measurement of inhibition of reduction of cytochrome c by O2- produced by the xanthine oxidase/xanthine reaction Laternula elliptica O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 24800, about, sequence calculation Laternula elliptica

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 manganese superoxide dismutase
-
 Laternula elliptica
1.15.1.1 MnSOD
-
 Laternula elliptica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 40
-
 30 min, purified enzyme, 50% activity remaining Laternula elliptica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 9
-
-
 Laternula elliptica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 8 10.5 50% of maximal activity at pH 8.0 and pH 10.5 Laternula elliptica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Laternula elliptica sequence calculation
-
 6.75