EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | additional information | activity increases up to 2.5fold during incubation at 50°C under H2 | Hydrogenophaga sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | O2 | enzyme is quite stable under air atmosphere, half-life of activity is ca. 48 h at 4°C, inactivation is irreversible | Hydrogenophaga sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.99.6 | 0.025 | - |
oxidized methylene blue | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 1.78 | - |
benzyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 12.5 | - |
methyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.99.6 | membrane | - |
Hydrogenophaga sp. | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | Fe | membrane-bound [NiFe]-hydrogenase exhibits prominent electron paramagnetic resonance signals originating from [3Fe4S]1 and [4Fe4S]1 clusters | Hydrogenophaga sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 37000 | - |
1 * 62000 + 1 * 37000, SDS-PAGE | Hydrogenophaga sp. |
1.12.99.6 | 62000 | - |
1 * 62000 + 1 * 37000, SDS-PAGE | Hydrogenophaga sp. |
1.12.99.6 | 100000 | - |
gel filtration | Hydrogenophaga sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.99.6 | Hydrogenophaga sp. | - |
AH-24 | - |
1.12.99.6 | Hydrogenophaga sp. AH-24 | - |
AH-24 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.99.6 | to homogeneity, by gel filtration, 133fold purified and 68.2% of yield relative to the membrane | Hydrogenophaga sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 110 | - |
purified enzyme | Hydrogenophaga sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.99.6 | H2 + oxidized benzyl viologen | - |
Hydrogenophaga sp. | H+ + reduced benzyl viologen | - |
? | |
1.12.99.6 | H2 + oxidized benzyl viologen | - |
Hydrogenophaga sp. AH-24 | H+ + reduced benzyl viologen | - |
? | |
1.12.99.6 | H2 + oxidized methyl viologen | - |
Hydrogenophaga sp. | H+ + reduced methyl viologen | - |
? | |
1.12.99.6 | H2 + oxidized methyl viologen | - |
Hydrogenophaga sp. AH-24 | H+ + reduced methyl viologen | - |
? | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. | H+ + reduced methylene blue | - |
? | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. AH-24 | H+ + reduced methylene blue | - |
? | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. | reduced methylene blue + H+ | - |
r | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. AH-24 | reduced methylene blue + H+ | - |
r | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. | reduced methylene blue | - |
r | |
1.12.99.6 | H2 + oxidized methylene blue | - |
Hydrogenophaga sp. AH-24 | reduced methylene blue | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.99.6 | heterodimer | 1 * 62000 + 1 * 37000, SDS-PAGE | Hydrogenophaga sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.99.6 | Mbh | - |
Hydrogenophaga sp. |
1.12.99.6 | membrane-bound [NiFe]-hydrogenase | - |
Hydrogenophaga sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 75 | - |
- |
Hydrogenophaga sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 50 | - |
under anaerobic conditions, retains full activity for over 5 h | Hydrogenophaga sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.99.6 | 20 | - |
methyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 240 | - |
benzyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 560 | - |
methylene blue | pH 7.0, 30°C | Hydrogenophaga sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 4 | - |
H2 evolution | Hydrogenophaga sp. |
1.12.99.6 | 8 | - |
H2 oxidation | Hydrogenophaga sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 3.5 | 9 | H2 evolution, pH 3.5 and 9.0: ca. 50% of maximal activity | Hydrogenophaga sp. |
1.12.99.6 | 5.5 | 9 | H2 oxidation, pH 5.5: 40% of maximal activity, pH 9: 60% of maximal activity | Hydrogenophaga sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.99.6 | 1.6 | - |
methyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 135 | - |
benzyl viologen | pH 7.0, 30°C | Hydrogenophaga sp. | |
1.12.99.6 | 22400 | - |
methylene blue | pH 7.0, 30°C | Hydrogenophaga sp. |