BRENDA - Enzyme Database

Molecular and functional characterization of the plastid-localized phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana

Prabhakar, V.; Loettgert, T.; Gigolashvili, T.; Bell, K.; Fluegge, U.I.; Haeusler, R.E.; FEBS Lett. 583, 983-991 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 cells
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.082
-
2-phospho-D-glycerate
at pH 8.0
Arabidopsis thaliana
4.2.1.11
0.149
-
2-phospho-D-glycerate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
0.18
-
phosphoenolpyruvate
at pH 8.0
Arabidopsis thaliana
4.2.1.11
0.39
-
3-phospho-D-glycerate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
0.534
-
phosphoenolpyruvate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
1.281
-
3-phospho-D-glycerate
at pH 8.0
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
plastid
-
Arabidopsis thaliana
9536
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
51500
-
calculated from amino acid sequence
Arabidopsis thaliana
4.2.1.11
61200
-
estimated from SDS-PAGE
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography, gel filtration
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
ENO1 is expressed in most heterotrophic tissues but not in the mesophyll of leaves
Arabidopsis thaliana
-
4.2.1.11
non-root-hair cell
-
Arabidopsis thaliana
-
4.2.1.11
trichome
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
703731
Arabidopsis thaliana
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
3-phospho-D-glycerate
-
703731
Arabidopsis thaliana
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
in the direction of 2-phospho-D-glycerate formation
Arabidopsis thaliana
4.2.1.11
8
-
in the direction of phosphoenolpyruvate formation
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 cells
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.082
-
2-phospho-D-glycerate
at pH 8.0
Arabidopsis thaliana
4.2.1.11
0.149
-
2-phospho-D-glycerate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
0.18
-
phosphoenolpyruvate
at pH 8.0
Arabidopsis thaliana
4.2.1.11
0.39
-
3-phospho-D-glycerate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
0.534
-
phosphoenolpyruvate
at pH 7.0
Arabidopsis thaliana
4.2.1.11
1.281
-
3-phospho-D-glycerate
at pH 8.0
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
plastid
-
Arabidopsis thaliana
9536
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
51500
-
calculated from amino acid sequence
Arabidopsis thaliana
4.2.1.11
61200
-
estimated from SDS-PAGE
Arabidopsis thaliana
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography, gel filtration
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
additional information
ENO1 is expressed in most heterotrophic tissues but not in the mesophyll of leaves
Arabidopsis thaliana
-
4.2.1.11
non-root-hair cell
-
Arabidopsis thaliana
-
4.2.1.11
trichome
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
703731
Arabidopsis thaliana
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
3-phospho-D-glycerate
-
703731
Arabidopsis thaliana
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7
-
in the direction of 2-phospho-D-glycerate formation
Arabidopsis thaliana
4.2.1.11
8
-
in the direction of phosphoenolpyruvate formation
Arabidopsis thaliana