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Literature summary extracted from

  • Prabhakar, V.; Loettgert, T.; Gigolashvili, T.; Bell, K.; Fluegge, U.I.; Haeusler, R.E.
    Molecular and functional characterization of the plastid-localized phosphoenolpyruvate enolase (ENO1) from Arabidopsis thaliana (2009), FEBS Lett., 583, 983-991.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli BL21 cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.082
-
 2-phospho-D-glycerate at pH 8.0 Arabidopsis thaliana
4.2.1.11 0.149
-
 2-phospho-D-glycerate at pH 7.0 Arabidopsis thaliana
4.2.1.11 0.18
-
 phosphoenolpyruvate at pH 8.0 Arabidopsis thaliana
4.2.1.11 0.39
-
 3-phospho-D-glycerate at pH 7.0 Arabidopsis thaliana
4.2.1.11 0.534
-
 phosphoenolpyruvate at pH 7.0 Arabidopsis thaliana
4.2.1.11 1.281
-
 3-phospho-D-glycerate at pH 8.0 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 plastid
-
 Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 51500
-
 calculated from amino acid sequence Arabidopsis thaliana
4.2.1.11 61200
-
 estimated from SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 Ni-NTA column chromatography, gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 additional information ENO1 is expressed in most heterotrophic tissues but not in the mesophyll of leaves Arabidopsis thaliana
-
4.2.1.11 non-root-hair cell
-
 Arabidopsis thaliana
-
4.2.1.11 trichome
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Arabidopsis thaliana phosphoenolpyruvate + H2O
-
 r
4.2.1.11 3-phospho-D-glycerate
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 ENO1
-
 Arabidopsis thaliana
4.2.1.11 phosphoenolpyruvate enolase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 7
-
 in the direction of 2-phospho-D-glycerate formation Arabidopsis thaliana
4.2.1.11 8
-
 in the direction of phosphoenolpyruvate formation Arabidopsis thaliana