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Literature summary extracted from

  • Poliak, P.; Van Hoewyk, D.; Obornik, M.; Zikova, A.; Stuart, K.D.; Tachezy, J.; Pilon, M.; Lukes, J.
    Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei (2010), FEBS J., 277, 383-393.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.7 cytoplasm
-
 Trypanosoma brucei 5737
-
2.8.1.7 cytosol
-
 Trypanosoma brucei 5829
-
2.8.1.7 mitochondrion
-
 Trypanosoma brucei 5739
-
2.8.1.7 nucleus
-
 Trypanosoma brucei 5634
-
4.4.1.16 cytoplasm
-
 Trypanosoma brucei 5737
-
4.4.1.16 nucleus
-
 Trypanosoma brucei 5634
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.7 Trypanosoma brucei
-
-
-
2.8.1.7 Trypanosoma brucei Q386Y7
-
-
4.4.1.16 Trypanosoma brucei
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.7 0.1
-
 knockdown of Nfs-like proteins, activity measured in mitochondrial fractions Trypanosoma brucei
2.8.1.7 0.2
-
 knockdown of selenocysteine lyase, activity measured in mitochondrial fractions Trypanosoma brucei
2.8.1.7 0.5
-
 knockdown of selenocysteine lyase, activity measured in cytosolic fractions Trypanosoma brucei
2.8.1.7 0.9
-
 activity measured in mitochondrial fractions Trypanosoma brucei
2.8.1.7 1
-
 knockdown of Nfs-like proteins, activity measured in cytosolic fractions Trypanosoma brucei
2.8.1.7 2.5
-
 activity measured in cytosolic fractions Trypanosoma brucei
4.4.1.16 200
-
 knockdown of Nfs-like proteins, activity measured in mitochondrial fractions Trypanosoma brucei
4.4.1.16 300
-
 knockdown of selenocysteine lyase, activity measured in mitochondrial fractions Trypanosoma brucei
4.4.1.16 600
-
 activity measured in mitochondrial fractions Trypanosoma brucei
4.4.1.16 1000
-
 knockdown of selenocysteine lyase, activity measured in cytosolic fractions Trypanosoma brucei
4.4.1.16 1200
-
 knockdown of Nfs-like proteins, activity measured in cytosolic fractions Trypanosoma brucei
4.4.1.16 1500
-
 activity measured in cytosolic fractions Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.7 L-cysteine + [enzyme]-cysteine
-
 Trypanosoma brucei L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.7 CysD
-
 Trypanosoma brucei
2.8.1.7 cysteine desulfurase
-
 Trypanosoma brucei
2.8.1.7 Nfs formerly IscS2 Trypanosoma brucei
4.4.1.16 SCL
-
 Trypanosoma brucei
4.4.1.16 selenocysteine lyase
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.7 25
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.7 7.4
-
 assay at Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.7 pyridoxal 5'-phosphate
-
 Trypanosoma brucei