EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.2.5 | modified N-terminal region, His-tagged version, expressed in Pichia pastoris | Mus musculus |
2.3.2.5 | modified N-terminal region, His-tagged version, expressed in Pichia pastoris | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.2.5 | C369A | improvement of enzyme secretion (expressed in Pichia pastoris) | Homo sapiens |
2.3.2.5 | I73N | artificial glycosylation site | Homo sapiens |
2.3.2.5 | I73N/C369A | further improvement of enzyme secretion (expressed in Pichia pastoris) | Homo sapiens |
2.3.2.5 | I74N | artificial glycosylation site | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.5 | benzimidazole | competitive | Homo sapiens | |
2.3.2.5 | benzimidazole | competitive | Mus musculus | |
2.3.2.5 | benzylimidazole | competitive | Homo sapiens | |
2.3.2.5 | benzylimidazole | competitive | Mus musculus | |
2.3.2.5 | cysteamine | - |
Mus musculus | |
2.3.2.5 | imidazole | - |
Homo sapiens | |
2.3.2.5 | imidazole | - |
Mus musculus | |
2.3.2.5 | L-glutaminyl-7-amido-4-methylcoumarin | substrate inhibition | Homo sapiens | |
2.3.2.5 | L-glutaminyl-beta-naphthylamide | substrate inhibition | Homo sapiens | |
2.3.2.5 | L-glutaminyl-beta-naphthylamide | substrate inhibition | Mus musculus | |
2.3.2.5 | methylimidazole | - |
Homo sapiens | |
2.3.2.5 | methylimidazole | - |
Mus musculus | |
2.3.2.5 | N,N-dimethylcysteamine | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.5 | 0.029 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.03 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.03 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.032 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.035 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.04 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.058 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.06 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.08 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.092 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.1 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.102 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.11 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.16 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.16 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.16 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.23 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.36 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.47 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.61 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.2.5 | Golgi complex | - |
Homo sapiens | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.5 | Homo sapiens | - |
- |
- |
2.3.2.5 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.5 | His-tagged version | Homo sapiens |
2.3.2.5 | hydrophobic interaction chromatography, ion exchange chromatography, gel filtration | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.5 | Gln-Gln | - |
Mus musculus | pGlu-Gln + NH3 | - |
? | |
2.3.2.5 | Gln-Gln | - |
Homo sapiens | pGlu-Gln + NH3 | - |
? | |
2.3.2.5 | Gln-Glu | - |
Mus musculus | pGlu-Glu + NH3 | - |
? | |
2.3.2.5 | Gln-Glu | - |
Homo sapiens | pGlu-Glu + NH3 | - |
? | |
2.3.2.5 | Gln-Gly | - |
Mus musculus | pGlu-Gly + NH3 | - |
? | |
2.3.2.5 | Gln-Gly | - |
Homo sapiens | pGlu-Gly + NH3 | - |
? | |
2.3.2.5 | Gln-Gly-Pro | - |
Mus musculus | pGlu-Gly-Pro + NH3 | - |
? | |
2.3.2.5 | Gln-Gly-Pro | - |
Homo sapiens | pGlu-Gly-Pro + NH3 | - |
? | |
2.3.2.5 | Gln-Phe-Ala | - |
Mus musculus | pGlu-Phe-Ala + NH3 | - |
? | |
2.3.2.5 | Gln-Phe-Ala | - |
Homo sapiens | pGlu-Phe-Ala + NH3 | - |
? | |
2.3.2.5 | Gln-Tyr-Ala | - |
Mus musculus | pGlu-Tyr-Ala + NH3 | - |
? | |
2.3.2.5 | Gln-Tyr-Ala | - |
Homo sapiens | pGlu-Tyr-Ala + NH3 | - |
? | |
2.3.2.5 | L-glutaminyl-7-amido-4-methylcoumarin | - |
Mus musculus | pGlu-7-amido-4-methylcoumarin + NH3 | - |
? | |
2.3.2.5 | L-glutaminyl-7-amido-4-methylcoumarin | - |
Homo sapiens | pGlu-7-amido-4-methylcoumarin + NH3 | - |
? | |
2.3.2.5 | L-glutaminyl-beta-naphthylamide | - |
Mus musculus | pGlu-beta-naphthylamide + NH3 | - |
? | |
2.3.2.5 | L-glutaminyl-beta-naphthylamide | - |
Homo sapiens | pGlu-beta-naphthylamide + NH3 | - |
? | |
2.3.2.5 | QEDL | - |
Mus musculus | pEEDL + NH3 | - |
? | |
2.3.2.5 | QEDL | - |
Homo sapiens | pEEDL + NH3 | - |
? | |
2.3.2.5 | QEYF | - |
Mus musculus | pEEYF + NH3 | - |
? | |
2.3.2.5 | QEYF | - |
Homo sapiens | pEEYF + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.5 | glutaminyl cyclase | isoenzyme | Mus musculus |
2.3.2.5 | glutaminyl cyclase | isoenzyme | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.5 | 1.07 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 1.65 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 2.6 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 2.7 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 3.3 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 3.4 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 4 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 4.57 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 6.4 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 6.4 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 6.98 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 7.5 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 7.7 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 7.79 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 8.66 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 11.4 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 11.78 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 17.48 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 22.9 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 24.1 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.2.5 | 7.5 | 8 | for kcat/KM | Mus musculus |
2.3.2.5 | 7.5 | 8 | for kcat/KM | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.5 | 0.0039 | - |
benzylimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.0062 | - |
benzylimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.027 | - |
N,N-dimethylcysteamine | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.052 | - |
methylimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.069 | - |
cysteamine | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.082 | - |
methylimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.103 | - |
imidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.124 | - |
benzimidazole | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 0.235 | - |
imidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 0.25 | - |
benzimidazole | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 1.57 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 3.55 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 4.47 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.5 | 9 | - |
Gln-Glu | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 9 | - |
Gln-Gly | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 16 | - |
Gln-Glu | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 28 | - |
Gln-Gly | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 38 | - |
Gln-Gly-Pro | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 54 | - |
Gln-Gln | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 55 | - |
QEDL | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 95 | - |
Gln-Gln | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 103 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 104 | - |
QEDL | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 111 | - |
Gln-Gly-Pro | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 117 | - |
Gln-Phe-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 123 | - |
QEYF | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 207 | - |
Gln-Tyr-Ala | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 229 | - |
L-glutaminyl-beta-naphthylamide | protein version: shortened N-terminus, I73N/C369A, His-tagged, pH 8.0, 30°C, similar value with other protein versions | Homo sapiens | |
2.3.2.5 | 311 | - |
L-glutaminyl-7-amido-4-methylcoumarin | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 394 | - |
Gln-Tyr-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 403 | - |
Gln-Phe-Ala | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 413 | - |
QEYF | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus | |
2.3.2.5 | 554 | - |
L-glutaminyl-beta-naphthylamide | protein expressed in Pichia pastoris, pH 8.0, 30°C | Mus musculus |