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Literature summary extracted from

  • Relat, J.; Pujol-Vidal, M.; Haro, D.; Marrero, P.F.
    A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme (2009), FEBS J., 276, 210-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 expressed in Pichia pastoris Homo sapiens
2.3.1.21 expressed in Pichia pastoris Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.21 D17E pig protein: Glu in this position Homo sapiens
2.3.1.21 E17D human protein: Asp in this position Sus scrofa
2.3.1.21 additional information deletion of residues 1-18, and deletion of residues 1-28, human-pig chimeras Sus scrofa
2.3.1.21 additional information pig-human chimeras Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA
-
 Homo sapiens
2.3.1.21 malonyl-CoA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.1976
-
 L-carnitine wild-type protein, mitochondrial preparation Sus scrofa
2.3.1.21 0.6059
-
 L-carnitine E17D mutant protein, mitochondrial preparation Sus scrofa
2.3.1.21 0.683
-
 L-carnitine wild-type protein, mitochondrial preparation Homo sapiens
2.3.1.21 0.7695
-
 L-carnitine D17E mutant protein, mitochondrial preparation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Homo sapiens
-
-
-
2.3.1.21 Sus scrofa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.21 0.00279
-
 wild-type protein, mitochondrial preparation Sus scrofa
2.3.1.21 0.00416
-
 chimeric protein: residues 1-128 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa
2.3.1.21 0.00443
-
 wild-type protein, mitochondrial preparation Homo sapiens
2.3.1.21 0.00449
-
 chimeric protein: residues 1-50 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa
2.3.1.21 0.00942
-
 protein with deletion of residue 1-28, mitochondrial preparation Sus scrofa
2.3.1.21 0.00943
-
 chimeric protein: residues 1-50 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens
2.3.1.21 0.01059
-
 chimeric protein: residues 1-128 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens
2.3.1.21 0.01528
-
 protein with deletion of residue 1-18, mitochondrial preparation Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 L-carnitine + palmitoyl-CoA
-
 Homo sapiens L-palmitoylcarnitine + CoA
-
 ?
2.3.1.21 L-carnitine + palmitoyl-CoA
-
 Sus scrofa L-palmitoylcarnitine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.21 carnitine palmitoyltransferase 1B
-
 Homo sapiens
2.3.1.21 carnitine palmitoyltransferase 1B
-
 Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.21 0.000096
-
 wild-type protein, 1 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.000117
-
 wild-type protein, 0.683 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.00019
-
 chimeric protein: residues 1-50 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.000246
-
 D17E mutant protein, 0.770 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.000279
-
 D17E mutant protein, 1 mM carnitine, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.000284
-
 E17D mutant protein, 0.606 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.000297
-
 E17D mutant protein, 1 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.000325
-
 chimeric protein: residues 1-128 of pig enzyme recombined with human enzyme, mitochondrial preparation Homo sapiens malonyl-CoA
2.3.1.21 0.000359
-
 chimeric protein: residues 1-128 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.000457
-
 chimeric protein: residues 1-50 of human enzyme recombined with pig enzyme, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.00055
-
 wild-type protein, 0.198 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.000804
-
 wild-type protein, 1 mM carnitine, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.03556
-
 protein with deletion of residue 1-18, mitochondrial preparation Sus scrofa malonyl-CoA
2.3.1.21 0.03919
-
 protein with deletion of residue 1-28, mitochondrial preparation Sus scrofa malonyl-CoA