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Literature summary extracted from

  • Stöveken, T.; Kalscheuer, R.; Steinbüchel, A.
    Both histidine residues of the conserved HHXXXDG motif are essential for wax ester synthase/acyl-CoA:diacylglycerol acyltransferase catalysis (2009), Eur. J. Lipid Sci. Technol., 111, 112-119.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 expressed in Escherichia coli Acinetobacter baylyi
2.3.1.75 gene atfA, expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3) Acinetobacter baylyi

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 D137A highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity Acinetobacter baylyi
2.3.1.20 G138A highly conserved residue in acyltransferases, mutation does not result in a significant decrease of activity Acinetobacter baylyi
2.3.1.20 H132L highly conserved residue in acyltransferases, mutation results in strongly decreased activity Acinetobacter baylyi
2.3.1.20 H132L/H133L highly conserved residues in acyltransferases Acinetobacter baylyi
2.3.1.20 H133L highly conserved residue in acyltransferases, residue essential for catalytic activity Acinetobacter baylyi
2.3.1.75 D137A the mutant shows slightly reduced activity compared to the wild-type enzyme Acinetobacter baylyi
2.3.1.75 G138A the mutant shows slightly reduced activity compared to the wild-type enzyme Acinetobacter baylyi
2.3.1.75 H132L the replacement of His132 leads to reduced enzyme activity Acinetobacter baylyi
2.3.1.75 H132L/H133L inactive mutant Acinetobacter baylyi
2.3.1.75 H133L the replacement of His133 leads to highly reduced enzyme activity Acinetobacter baylyi

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Acinetobacter baylyi
-
-
-
2.3.1.75 Acinetobacter baylyi
-
 gene atfA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 cation-exchange chromatography, hydrophobic interaction chromatography, anion-exchange chromatography Acinetobacter baylyi
2.3.1.75 recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange and hydrophobic interaction chromatography, followed by anion exchange chromatography Acinetobacter baylyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.75 0.00000005
-
 recombinant mutant H133L Acinetobacter baylyi
2.3.1.75 0.0000005
-
 recombinant mutant H132L Acinetobacter baylyi
2.3.1.75 0.0000414
-
 recombinant mutant D137A Acinetobacter baylyi
2.3.1.75 0.0000464
-
 recombinant wild-type enzyme Acinetobacter baylyi
2.3.1.75 0.0000517
-
 recombinant mutant G138A Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 palmitoyl-CoA + 1,2-dipalmitin
-
 Acinetobacter baylyi CoA + tripalmitin
-
 ?
2.3.1.75 additional information WS/DGAT is a very unspecific enzyme and accepts a wide variety of hydrophobic substrates, although also several compounds, e.g. hydroxylamine, sugars, and astaxanthine, do not serve as substrates, substrate specificity, overview. The highly conserved HHXXXDG motif is located at the enzyme's active site, it possesses two histidine residues that are essential for catalysis Acinetobacter baylyi ?
-
 ?
2.3.1.75 palmitoyl-CoA + 1,2-dipalmitin
-
 Acinetobacter baylyi ?
-
 ?
2.3.1.75 palmitoyl-CoA + 1-hexadecanol
-
 Acinetobacter baylyi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
 Acinetobacter baylyi
2.3.1.75 ATF
-
 Acinetobacter baylyi
2.3.1.75 wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
 Acinetobacter baylyi
2.3.1.75 WS/DGAT
-
 Acinetobacter baylyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.75 35
-
 assay at Acinetobacter baylyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.75 7.4
-
 assay at Acinetobacter baylyi