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Literature summary extracted from

  • Nakano, M.; Kelly, E.J.; Rettie, A.E.
    Expression and characterization of CYP4V2 as a fatty acid omega-hydroxylase (2009), Drug Metab. Dispos., 37, 2119-2122.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.80 expressed from pFastbacCYP4V2HIS vector in Escherichia coli DH10Bac. Hexahistidine-tagged CYP4V2 is expressed in Sf-9 cells after infection with the recombinant baculovirus Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.80 (E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide HET0016 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.80 0.065
-
 myristic acid
-
 Homo sapiens
1.14.14.80 0.14
-
 lauric acid
-
 Homo sapiens
1.14.14.80 0.43
-
 palmitic acid
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.80 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.80 55000
-
 by Coomassie staining and by immunoblotting Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.80 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.80 eye
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.80 lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 additional information octanoic acid is not detectably omega-hydroxylated by CYP4V2 Homo sapiens ?
-
 ?
1.14.14.80 myristic acid + [reduced NADPH-hemoprotein reductase] + O2 CYP4V2 is a selective omega-hydroxylase of saturated, medium-chain fatty acids with relatively high catalytic efficiency toward myristic acid Homo sapiens 12-hydroxymyristic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.80 palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.80 CYP4V2
-
 Homo sapiens
1.14.14.80 Fatty acid omega-hydroxylase
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.14.80 0.000038
-
-
 Homo sapiens (E)-N'-(4-butyl-2-methylphenyl)-N-hydroxyformimidamide

General Information

EC Number General Information Comment Organism
1.14.14.80 physiological function defective omega-oxidation of ocular fatty acids/lipids secondary to mutations in the CYP4V2 gene may be a plausible mechanism underlying Bietti's crystalline dystrophy Homo sapiens