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Literature summary extracted from

  • Lin, L.L.; Yang, L.Y.; Hu, H.Y.; Lo, H.F.
    Influence of N-terminal truncations on the functional expression of Bacillus licheniformis gamma-glutamyltranspeptidase in recombinant Escherichia coli (2008), Curr. Microbiol., 57, 603-608.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expression in Escherichia coli Bacillus licheniformis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 additional information expression of full length gene and six truncations lacking 36, 129, 132, 135, 144, and 174 bp, respectively, at the 5' end in Escherichia coli. Mutant proteins derived from genes lacking 135 and 144 bp show no enzymatic activity. Mutants derived of truncations of 36. 129, 132 process autocatalytically their precursors into alpha- und beta-subunits at 4°C Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus licheniformis
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-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 0.2
-
 mutant derived from truncation of 132 bp at the 5' end, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 0.8
-
 mutant derived from truncation of 129 bp at the 5' end, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 1.3
-
 mutant derived from truncation of 36 bp at the 5' end, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 51.9
-
 wild-type, pH 8.0, 40°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly-Gly
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 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
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