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Literature summary extracted from

  • Yamamoto, K.; Nagaoka, S.; Banno, Y.; Aso, Y.
    Biochemical properties of an omega-class glutathione S-transferase of the silkmoth, Bombyx mori (2009), Comp. Biochem. Physiol. C, 149, 461-467.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 isozyme GSTO, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional overexpression in Escherichia coli as soluble protein Bombyx mori
2.5.1.18 sequence comparisons and phylogenetic analysis Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 deltamethrin 41% inhibition at 1 mM; 54% inhibition at 1 mM Bombyx mori
2.5.1.18 fenitrothion 50% inhibition at 1 mM; 56% inhibition at 1 mM Bombyx mori
2.5.1.18 additional information the enzyme is completely resistant against insecticides fenitrothion, deltamethrin, and permethrin, in vivo toxicity, overview Bombyx mori
2.5.1.18 permethrin 24% inhibition at 1 mM; 33% inhibition at 1 mM Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information kinetics Bombyx mori
2.5.1.18 0.006
-
 4-hydroxynonenal GSTO Bombyx mori
2.5.1.18 0.14
-
 4-hydroxynonenal GSTD Bombyx mori
2.5.1.18 0.48
-
 1-chloro-2,4-dinitrobenzene GSTD Bombyx mori
2.5.1.18 0.67
-
 1-chloro-2,4-dinitrobenzene GSTO Bombyx mori
2.5.1.18 1.6
-
 1-chloro-2,4-dinitrobenzene GSTS Bombyx mori
2.5.1.18 2.1
-
 4-hydroxynonenal GSTS Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Bombyx mori 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 23338
-
 x * 23338, GSTO, sequence calculation Bombyx mori
2.5.1.18 24225
-
 x * 24225, GSTO, sequence calculation Bombyx mori
2.5.1.18 29806
-
 x * 29806, GSTO, sequence calculation Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bombyx mori A8R5V3 omega-GST
-
2.5.1.18 Bombyx mori Q5CCJ4 sigma-GST
-
2.5.1.18 Bombyx mori Q60GK5 delta-GST
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant isozyme GSTO from Escherichia coli to homogeneity by ammonium sulfate fractionation, anion exchange chromatography and gel filtration Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 fat body
-
 Bombyx mori
-
2.5.1.18 larva fifth-instar larvae Bombyx mori
-
2.5.1.18 midgut
-
 Bombyx mori
-
2.5.1.18 silk gland low expression level Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Bombyx mori S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 4-hydroxynonenal + glutathione
-
 Bombyx mori ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Bombyx mori S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-hydroxynonenal a lipid peroxidation product Bombyx mori ?
-
 ?
2.5.1.18 glutathione + 4-nitrophenyl acetate
-
 Bombyx mori S-(4-nitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Bombyx mori ?
-
 ?
2.5.1.18 additional information the enzyme shows high affinity for organophosphorus insecticide Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 23338, GSTO, sequence calculation Bombyx mori
2.5.1.18 ? x * 24225, GSTO, sequence calculation Bombyx mori
2.5.1.18 ? x * 29806, GSTO, sequence calculation Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 delta-class glutathione S-transferase
-
 Bombyx mori
2.5.1.18 glutathione S-transferase
-
 Bombyx mori
2.5.1.18 GSTD
-
 Bombyx mori
2.5.1.18 GSTO
-
 Bombyx mori
2.5.1.18 GSTS
-
 Bombyx mori
2.5.1.18 omega-class glutathione S-transferase
-
 Bombyx mori
2.5.1.18 sigma-class glutathione S-transferase
-
 Bombyx mori
2.5.1.18 sigma-class GST
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 40
-
 purified GSTS, stable up to Bombyx mori
2.5.1.18 50
-
 purified GSTD, stable up to Bombyx mori
2.5.1.18 50
-
 purified GSTO, stable up to Bombyx mori
2.5.1.18 60
-
 purified GSTO, 50% inhibitioin Bombyx mori
2.5.1.18 80
-
 purified GSTO, inactivation Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 4 9 GSTD Bombyx mori
2.5.1.18 7 8 GSTO Bombyx mori
2.5.1.18 8
-
 GSTS Bombyx mori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 2 10 activity range, about 10% of maximal activity at pH 2.0 and pH 10.0 Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 2 12 GSTO shows 75% of maximal activity at pH 2.0 and pH 12.0 after 24 h at 4°C Bombyx mori
2.5.1.18 4 11 GSTS Bombyx mori
2.5.1.18 5 11 GSTD Bombyx mori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Bombyx mori GSTS, sequence calculation
-
 5.79
2.5.1.18 Bombyx mori GSTO, sequence calculation
-
 6.01
2.5.1.18 Bombyx mori GSTD, sequence calculation
-
 8.35

General Information

EC Number General Information Comment Organism
2.5.1.18 physiological function bmGSTO might be involved in the increase in level of insecticide resistance for lepidopteran insects Bombyx mori