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Literature summary extracted from

  • Plese, B.; Schroeder, H.C.; Grebenjuk, V.A.; Wegener, G.; Brandt, D.; Natalio, F.; Mueller, W.E.
    Strombine dehydrogenase in the demosponge Suberites domuncula: characterization and kinetic properties of the enzyme crucial for anaerobic metabolism (2009), Comp. Biochem. Physiol. B, 154, 102-107.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.22 pyruvate inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM Suberites domuncula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.22 0.34
-
 pyruvate pH 7.0, 30°C Suberites domuncula
1.5.1.22 0.34
-
 pyruvate determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0) Suberites domuncula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.22 35000
-
 native PAGE Suberites domuncula
1.5.1.22 35000
-
 semi-native PAGE Suberites domuncula
1.5.1.22 75000
-
 native PAGE Suberites domuncula
1.5.1.22 75000
-
 semi-native PAGE two bands at 35 kDa and 75 kDa are visible Suberites domuncula

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.22 Suberites domuncula B1GT63
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.22 0.079
-
-
 Suberites domuncula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.22 Gly + pyruvate + NADH the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment Suberites domuncula D-Strombine + NAD+ + H2O
-
 ?
1.5.1.22 glycine + pyruvate + NADH enzyme is specific for glycine Suberites domuncula D-strombine + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.22 dimer native PAGE and semi-native PAGE, 2 * 35000 Da Suberites domuncula
1.5.1.22 dimer native and semi-native PAGE analysis, 2 * 35000 Suberites domuncula

Synonyms

EC Number Synonyms Comment Organism
1.5.1.22 StDH shows 68% sequence identity with the tauropine dehydrogenase protein from the marine demosponge Halichondria japonica Suberites domuncula
1.5.1.22 strombine dehydrogenase
-
 Suberites domuncula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.22 30
-
 assay at Suberites domuncula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.22 7
-
 assay at Suberites domuncula
1.5.1.22 7
-
 assay at and optimal pH Suberites domuncula

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.22 NADH
-
 Suberites domuncula
1.5.1.22 NADH enzyme shows an absolute requirement for NADH Suberites domuncula