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Literature summary extracted from

  • Yang, L.; Zou, H.; Zhu, H.; Ruppert, M.; Gong, J.; Stoeckigt, J.
    Improved expression of His(6)-tagged strictosidine synthase cDNA for chemo-enzymatic alkaloid diversification (2010), Chem. Biodivers., 7, 860-870.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2 expression in Escherichia coli, coexpression with chaerone pG-Tf2 Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.2 additional information
-
 values between 36000 and 41000, dependent on expression system Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Rauvolfia serpentina P68175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2 by Ni-NTA affinity chromatography Rauvolfia serpentina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.2 additional information
-
 activities between 14.29 nKat/l and 67.15 nKat/l, depending on expression system, increased activity in presence of chaperone pG-Tf2 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 7-azatryptamine + secologanin assay at pH 7.0, 28°C, reaction stopped by adding methanol Rauvolfia serpentina 3-alpha(S)-12-azastrictosidine
-
 ?
4.3.3.2 tryptamine + secologanin assay at pH 7.0, 28°C, reaction stopped by adding methanol Rauvolfia serpentina 3-alpha(S)-strictosidine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 STR1
-
 Rauvolfia serpentina
4.3.3.2 stricotsidine synthase
-
 Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.2 28
-
 assay at Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 7
-
 assay at Rauvolfia serpentina

General Information

EC Number General Information Comment Organism
4.3.3.2 physiological function involved in biosynthesis of monoterpenoid indole alkaloids Rauvolfia serpentina