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Literature summary extracted from

  • Roosild, T.P.; Castronovo, S.; Fabbiani, M.; Pizzorno, G.
    Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy (2009), BMC Struct. Biol., 9, 14.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 expression of the N-terminally His6-tagged UPP1 in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 free enzyme and enzyme in complex with inhibitor 5-benzylacyclouridine, 3 mg/ml protein in absence or presence of 1 mM inhibitor from 17% PEG 3350, 100 mM Bis-Tris buffer, pH 5.5, 300 mM KCl, and 30 mM MgCl2, or for the ligand-free enzyme crystals from 1.2 M (NH4)2SO4, 100 mM Bis-Tris buffer pH 5.5, with 1-2% MPD, with 2-3 mg/ml protein, X-ray diffraction structure determination and analysis at 2.3 A and 1.9 A resolution, respectively Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 5-benzylacyclouridine an UPP1-specific inhibitor. Binding of the inhibitor to the UPP1 active site, structure, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 additional information Homo sapiens UPP catalyzes the reversible phosphorolysis of uracil ribosides and analogous compounds to their respective nucleobases and ribose-1-phosphate, structural mechanisms, overview ?
-
 ?
2.4.2.3 uridine + phosphate Homo sapiens
-
 uracil + alpha-D-ribose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Homo sapiens Q16831 isozyme UPP1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 recombinant N-terminally His6-tagged UPP1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 additional information UPP catalyzes the reversible phosphorolysis of uracil ribosides and analogous compounds to their respective nucleobases and ribose-1-phosphate, structural mechanisms, overview Homo sapiens ?
-
 ?
2.4.2.3 uridine + phosphate
-
 Homo sapiens uracil + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.3 dimer isozyme UPP1, the N-terminus of the protein forms a strand-turn-strand structure bracketed by two short helices, structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 UPP1
-
 Homo sapiens
2.4.2.3 uridine phosphorylase 1
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.2.3 metabolism uridine phosphorylase is a ubiquitous enzyme involved in pyrimidine salvage and maintenance of uridine homeostasis Homo sapiens
2.4.2.3 physiological function uridine phosphorylase is a ubiquitous enzyme involved in pyrimidine salvage and maintenance of uridine homeostasis. UPP1 plays a role in the activation of pyrimidine nucleoside analogues used in chemotherapy, such as 5-fluorouracil and its prodrug, capecitabine Homo sapiens