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Literature summary extracted from
- Characterization of a recombinant L-fucose isomerase from Caldicellulosiruptor saccharolyticus that isomerizes L-fucose, D-arabinose, D-altrose, and L-galactose (2010), Biotechnol. Lett., 32, 299-304.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.3 | into the vector pGEM-T Easy, and subsequently into the vector pET15b for expression in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | into the vector pGEM-T Easy, and subsequently into the vector pET15b for expression in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.3 | 61 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus |  |
| 5.3.1.3 | 152 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus | |
| 5.3.1.25 | 141 | - |
L-fucose | - |
Caldicellulosiruptor saccharolyticus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.3 | Mn2+ | - |
Caldicellulosiruptor saccharolyticus | |
| 5.3.1.25 | Mn2+ | - |
Caldicellulosiruptor saccharolyticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.3 | 68000 | - |
determined by SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 5.3.1.3 | 68110 | - |
theoretical | Caldicellulosiruptor saccharolyticus |
| 5.3.1.3 | 204000 | - |
homotrimer, determined by gel filtration | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 68000 | - |
determined by SDS-PAGE | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 68110 | - |
theoretical | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 204000 | - |
homotrimer, determined by gel filtration | Caldicellulosiruptor saccharolyticus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.3 | D-altrose | Caldicellulosiruptor saccharolyticus | - |
D-psicose | - |
? | |
| 5.3.1.3 | D-arabinose | Caldicellulosiruptor saccharolyticus | - |
D-ribulose | - |
? | |
| 5.3.1.3 | L-galactose | Caldicellulosiruptor saccharolyticus | - |
L-tagatose | - |
? | |
| 5.3.1.25 | L-Fucose | Caldicellulosiruptor saccharolyticus | - |
L-Fuculose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.3 | Caldicellulosiruptor saccharolyticus | A4XJ56 | - |
- |
| 5.3.1.25 | Caldicellulosiruptor saccharolyticus | A4XJ56 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.3 | on a His-Trap HP column | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | on a His-Trap HP column | Caldicellulosiruptor saccharolyticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.3 | 2 | - |
substrate L-galactose | Caldicellulosiruptor saccharolyticus |
| 5.3.1.3 | 15 | - |
substrate D-altrose | Caldicellulosiruptor saccharolyticus |
| 5.3.1.3 | 62 | - |
substrate D-arabinose | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 1.4 | - |
purification step crude extract, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 10 | - |
purification step heat treatment, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | 76 | - |
purification step His-Trap column, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.3 | D-altrose | - |
Caldicellulosiruptor saccharolyticus | D-psicose | - |
? | |
| 5.3.1.3 | D-arabinose | - |
Caldicellulosiruptor saccharolyticus | D-ribulose | - |
? | |
| 5.3.1.3 | L-galactose | - |
Caldicellulosiruptor saccharolyticus | L-tagatose | - |
? | |
| 5.3.1.25 | L-Fucose | - |
Caldicellulosiruptor saccharolyticus | L-Fuculose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.3 | homotrimer | 3 * 68000 Da | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | homotrimer | 3 * 68000 Da | Caldicellulosiruptor saccharolyticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.3 | D-Arabinose isomerase | L-fucose isomerase, bifunctional enzyme | Caldicellulosiruptor saccharolyticus |
| 5.3.1.25 | L-Fucose isomerase | D-arabinose isomerase, bifunctional enzyme | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.25 | 75 | - |
L-fucose isomerization | Caldicellulosiruptor saccharolyticus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.3 | 0.0287 | - |
D-altrose | - |
Caldicellulosiruptor saccharolyticus | |
| 5.3.1.3 | 0.1823 | - |
D-arabinose | - |
Caldicellulosiruptor saccharolyticus | |
| 5.3.1.25 | 0.1985 | - |
L-fucose | - |
Caldicellulosiruptor saccharolyticus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.25 | 7 | - |
L-fucose isomerization | Caldicellulosiruptor saccharolyticus |
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