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Literature summary extracted from

  • Deog, K.; Jin, S.; Kiyoshi, H.
    Construction and characterization of novel chimeric beta-glucosidases with Cellvibrio gilvus (CG) and Thermotoga maritima (TM) by overlapping PCR (2009), Biotechnol. Bioprocess Eng., 14, 266-273.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima
3.2.1.21 expressed in Escherichia coli BL21(DE3) cells Cellulomonas gilvus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.0039
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 0.0082
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 0.0082
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 0.012
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 0.012
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 0.44
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 80000
-
 chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE Thermotoga maritima
3.2.1.21 80000
-
 chimeric beta-glucosidases No. 6 and No. 8 type, SDS-PAGE Cellulomonas gilvus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Cellulomonas gilvus
-
-
-
3.2.1.21 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 Ni-NTA resin column chromatography and Mono-S column chromatography Thermotoga maritima
3.2.1.21 Ni-NTA resin column chromatography and Mono-S column chromatography Cellulomonas gilvus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.14
-
 chimeric beta-glucosidase No.8, crude extract, at 30°C Thermotoga maritima
3.2.1.21 0.14
-
 chimeric beta-glucosidase No.8, crude extract, at 30°C Cellulomonas gilvus
3.2.1.21 0.17
-
 chimeric beta-glucosidase No.6, crude extract, at 30°C Thermotoga maritima
3.2.1.21 0.17
-
 chimeric beta-glucosidase No.6, crude extract, at 30°C Cellulomonas gilvus
3.2.1.21 4.53
-
 chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C Thermotoga maritima
3.2.1.21 4.53
-
 chimeric beta-glucosidase No.8, after 32.35fold purification, at 30°C Cellulomonas gilvus
3.2.1.21 5.55
-
 chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C Thermotoga maritima
3.2.1.21 5.55
-
 chimeric beta-glucosidase No.6, after 32.65fold purification, at 30°C Cellulomonas gilvus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga maritima 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas gilvus 4-nitrophenol + D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bgl
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 30
-
 optimum temperature of the wild type enzyme Cellulomonas gilvus
3.2.1.21 60
-
 optimum temperature of No.6 and No.8 chimeric beta-glucosidases Thermotoga maritima
3.2.1.21 60
-
 optimum temperature of No.6 and No.8 chimeric beta-glucosidases Cellulomonas gilvus
3.2.1.21 70
-
 optimum temperature of the wild type enzyme Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 41 70 chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C above which activity decreases, the heat stability of the wild type enzyme is at 41°C Cellulomonas gilvus
3.2.1.21 60 87 chimeric beta-glucosidase No. 6 is stable up to 60°C, chimeric beta-glucosidase No. 8 is stable up to 70°C, above which activity decreases, the heat stability of the wild type enzyme is at 41°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 3.84
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 3.84
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 5.62
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 5.62
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 6.4
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 42.2
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 3
-
 the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used Thermotoga maritima
3.2.1.21 3
-
 the optimum pH of No. 6 chimeric beta-glucosidase is 3.0 in formate buffer, showing varying maximum activity according to the buffer used Cellulomonas gilvus
3.2.1.21 4.5
-
 the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 Thermotoga maritima
3.2.1.21 4.5
-
 the optimum pH of No. 8 chimeric beta-glucosidase is 4.5 Cellulomonas gilvus
3.2.1.21 5
-
 the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used Thermotoga maritima
3.2.1.21 5
-
 the optimum pH of No. 6 chimeric beta-glucosidase is 5.0 in succinate buffer, showing varying maximum activity according to the buffer used Cellulomonas gilvus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 95.9
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 468
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 468
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.6, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 468
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima
3.2.1.21 468
-
 4-nitrophenyl-beta-D-glucopyranoside chimeric beta-glucosidase No.8, in 50 mM MOPS buffer (pH 6.5), at 30°C Cellulomonas gilvus
3.2.1.21 1640
-
 4-nitrophenyl-beta-D-glucopyranoside wild type enzyme, in 50 mM MOPS buffer (pH 6.5), at 30°C Thermotoga maritima