EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.5.14 | biotechnology | multi-walled carbon nanotubes synthesized on platinum plate (MWCNTs/Pt) electrode are immediately immersed into solutions of FDH to immobilize the enzyme onto electrode surfaces. Thereafter, a well-defined catalytic oxidation current based on FDH is observed from ca. -0.15V, which is close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration is up to ca. 40 mmol/dm3, and the apparent Michaelis-Menten constant is evaluated to be 11 mmol/l. The obtained results are useful in applications to prepare the third-generation biosensors and other future bioelectrochemical devices | Gluconobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.14 | 11 | - |
D-fructose | Km is determined using FDH adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode | Gluconobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.14 | Gluconobacter sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.14 | D-fructose + acceptor | - |
Gluconobacter sp. | 5-dehydro-D-fructose + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.14 | FDH | - |
Gluconobacter sp. |
1.1.5.14 | fructose dehydrogenase | - |
Gluconobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.14 | 5 | - |
assay at | Gluconobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.14 | heme c | - |
Gluconobacter sp. |