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Literature summary extracted from

  • Yun, C.S.; Hasegawa, H.; Nanamiya, H.; Terakawa, T.; Tozawa, Y.
    Novel bacterial N-acetyltransferase gene for herbicide detoxification in land plants and selection maker in plant transformation (2009), Biosci. Biotechnol. Biochem., 73, 1000-1006.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.183 agriculture creation of herbicide resistant plants Nocardia sp. AB2253
2.3.1.183 biotechnology creation of herbicide resistant plants Nocardia sp. AB2253

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.183 expressed as chimeric protein in Escherichia coli and in pea chloroplasts after transformation with Agrobacterium tumefaciens Nocardia sp. AB2253

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.183 2.2
-
 L-phosphinothricin
-
 Nocardia sp. AB2253
2.3.1.183 14.31
-
 methionine sulfone
-
 Nocardia sp. AB2253

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.183 19600
-
 SDS-PAGE Nocardia sp. AB2253

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.183 Nocardia sp. AB2253 B0I560 soil isolate
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.183 acetyl-CoA + 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Nocardia sp. AB2253 CoA + 5-thio-2-nitrobenzoic acid
-
 ?
2.3.1.183 acetyl-CoA + L-phosphinothricin
-
 Nocardia sp. AB2253 CoA + L-N-acetylphosphinothricin
-
 ir
2.3.1.183 acetyl-CoA + methionine sulfone
-
 Nocardia sp. AB2253 CoA + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.183 MAT
-
 Nocardia sp. AB2253
2.3.1.183 methionine sulfone N-acetyltransferase
-
 Nocardia sp. AB2253
2.3.1.183 phosphinothricin-N-acetyltransferase
-
 Nocardia sp. AB2253

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.183 8.5
-
 in Tris buffer Nocardia sp. AB2253

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.183 7 9.5 Catalytic activity reaches a maximum at pH 8 to pH 8.5. In Tris buffer at pH 7 and pH 9.5 20-30% catalytic activity remains. In phosphate buffer, the activity at pH 7 and pH 6.5 is 15% and 10%, respectively. At pH 6 the enzyme shows no activity. Nocardia sp. AB2253

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.183 acetyl-CoA
-
 Nocardia sp. AB2253

General Information

EC Number General Information Comment Organism
2.3.1.183 metabolism detoxification of the nonselective herbicide phosphinothricin, commercial named Roundup Ready, Finale or Basta Nocardia sp. AB2253