Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhou, H.; Liao, X.; Wang, T.; Du, G.; Chen, J.
    Enhanced l-phenylalanine biosynthesis by co-expression of pheA(fbr) and aroF(wt) (2010), Biores. Technol., 101, 4151-4156.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.51 industry overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis, which is an important prerequisite for improved production of L-phenylalanine with engineered strains Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.51 expressed in different engineered Escherichia coli WSH-Z06 strains (pAP-B, pAP-B01, pAP-B02 and pAP-B03) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.51 T326P mutant harboring pheAfbr retains more than 70% of CM and PDT activities even in the presence of 200 mM L-phenylalanine, has potential in overproduction of L-phenylalanine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.51 L-phenylalanine feedback inhibition. PDT of Escherichia coli WSH-Z06 is almost completely inhibited by 10 mM L-phenylalanine (92.9% activity loss). PDT of Escherichia coli WSH-Z06 (pAP-B03) exhibits strong resistance to 200 mM L-phenylalanine, manifested by the high residual activities (10 mM L-phenylalanine with only 7.1% loss of activity) Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.51 43100
-
 x * 43100, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Escherichia coli C7EXK8 the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06
-
4.2.1.51 Escherichia coli K06 C7EXK8 the enzyme possesses prephenate dehydratase (EC 4.2.1.51) and chorismate mutase activity (EC 5.4.99.5); K06
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.51 3100
-
 strain WSH-Z06 (pAP-B) Escherichia coli
4.2.1.51 18000
-
 strain WSH-Z06 (pAP-B03) Escherichia coli
4.2.1.51 18600
-
 strain WSH-Z06 (pAP-B01) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
 Escherichia coli phenylpyruvate + H2O + CO2
-
 ?
4.2.1.51 prephenate
-
 Escherichia coli K06 phenylpyruvate + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.51 ? x * 43100, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.51 chorismate mutase-prephenate dehydratase the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) Escherichia coli
4.2.1.51 CM–PDT the enzyme also possesses chorismate mutase activity (EC 5.4.99.5) Escherichia coli
4.2.1.51 PheA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
4.2.1.51 malfunction use of a T326P mutation in the regulation domain of pheA (pheAfbr) for L-phenylalanine production. Overexpression of pheAfbr and/or wild-type aroF is helpful for the enhancement of the key enzymes in L-phenylalanine biosynthesis Escherichia coli