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Literature summary extracted from

  • Singh, R.; Vadlani, P.; Harrison, M.; Bennett, G.; San, K.
    Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients (2008), Bioprocess Biosyst. Eng., 31, 299-306.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.84 biotechnology a low-cost fermentation process for isoamyl acetate biosynthesis by overexpressing the yeast alcohol acetyl-transferase AFT1 in Escherichia coli is developed Saccharomyces cerevisiae
2.3.1.84 biotechnology a low-cost fermentation process for isoamyl acetate biosynthesis by overexpressing the yeast alcohol acetyl-transferase AFT2 in Escherichia coli is developed Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.84 a pPTB construct is used, expression experiments are performed in Escherichia coli cells Saccharomyces cerevisiae
2.3.1.84 into the pBAD-TOPO vector, furthermore a pTRC construct is used, expression experiments are performed in Escherichia coli cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.84 acetyl-CoA + isoamyl alcohol Saccharomyces cerevisiae
-
 CoA + isoamyl acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.84 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.84 acetyl-CoA + isoamyl alcohol
-
 Saccharomyces cerevisiae CoA + isoamyl acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.84 AFT1
-
 Saccharomyces cerevisiae
2.3.1.84 AFT2
-
 Saccharomyces cerevisiae
2.3.1.84 alcohol acetyl-transferase 1
-
 Saccharomyces cerevisiae
2.3.1.84 alcohol acetyl-transferase 2
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.84 acetyl-CoA
-
 Saccharomyces cerevisiae