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Literature summary extracted from

  • Yamamura, A.; Ichimura, T.; Kamekura, M.; Mizuki, T.; Usami, R.; Makino, T.; Ohtsuka, J.; Miyazono, K.; Okai, M.; Nagata, K.; Tanokura, M.
    Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases (2009), Biophys. J., 96, 4692-4700.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer (pH 7.6), 18% (w/v) PEG 400, and 10 mM EDTA, at 20°C Haloarcula sinaiiensis
2.7.4.6 sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer (pH 7.6), 18% (w/v) PEG 400, and 10 mM EDTA, at 20°C Haloarcula quadrata

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 R31C the mutation destabilizes the hexameric assembly, and causes dissociation to less active dimers at low salt concentrations Haloarcula quadrata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Na+ optimal NaCl concentration is 1 M Haloarcula quadrata
2.7.4.6 Na+ optimal NaCl concentration is 2 M Haloarcula sinaiiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 42000
-
 in the presence of 0.5 M NaCl, sedimentation velocity Haloarcula sinaiiensis
2.7.4.6 104000
-
 in the presence of 2 M NaCl, sedimentation velocity Haloarcula quadrata
2.7.4.6 111000
-
 in the presence of 2 M NaCl, sedimentation velocity Haloarcula sinaiiensis
2.7.4.6 114000
-
 in the presence of 0.5 M NaCl, sedimentation velocity Haloarcula quadrata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Haloarcula sinaiiensis
-
 ADP + NTP
-
 ?
2.7.4.6 ATP + NDP Haloarcula quadrata
-
 ADP + NTP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Haloarcula quadrata
-
-
-
2.7.4.6 Haloarcula sinaiiensis Q401C6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Haloarcula sinaiiensis
2.7.4.6 Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Haloarcula quadrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ADP + GTP
-
 Haloarcula sinaiiensis ATP + GDP
-
 ?
2.7.4.6 ADP + GTP
-
 Haloarcula quadrata ATP + GDP
-
 ?
2.7.4.6 ATP + NDP
-
 Haloarcula sinaiiensis ADP + NTP
-
 ?
2.7.4.6 ATP + NDP
-
 Haloarcula quadrata ADP + NTP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer present as a hexamer under a wide range of salt concentrations (0.2-4 M NaCl) Haloarcula sinaiiensis
2.7.4.6 hexamer present as a hexamer under a wide range of salt concentrations (0.2-4 M NaCl) Haloarcula quadrata

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDK
-
 Haloarcula sinaiiensis
2.7.4.6 NDK
-
 Haloarcula quadrata
2.7.4.6 nucleoside diphosphate kinase
-
 Haloarcula sinaiiensis
2.7.4.6 nucleoside diphosphate kinase
-
 Haloarcula quadrata

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Haloarcula sinaiiensis
2.7.4.6 ATP
-
 Haloarcula quadrata