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Literature summary extracted from

  • Kitahama, Y.; Nakamura, M.; Yoshida, Y.; Aoyama, Y.
    The construction and characterization of self-sufficient lanosterol 14-demethylase fusion proteins consisting of yeast CYP51 and its reductase (2009), Biol. Pharm. Bull., 32, 558-563.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 CYP51 cDNA cloned with pBluescript KS-. Self sufficient lanosterol 14-demethylase fusion proteins by using yeast CYP51 and P450 reductase, expressed in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 ketoconazole
-
Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.154 0.004
-
lanosterol fusion protein CYP51 (F1) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T, and DELTA41 P450 reductase Saccharomyces cerevisiae
1.14.14.154 0.0044
-
lanosterol fusion protein CYP51 (F2) consisting of the ORF of modified CYP51, a linker region containing the amino acid sequence S-T-E-Q-S-A-K-K-V-R-K-K-A, and DELTA55 P450 reductase Saccharomyces cerevisiae
1.14.14.154 0.005
-
lanosterol ORF of modified CYP51 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Saccharomyces cerevisiae P10614
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.154 to homogeneity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + NADPH + O2 artificial fused enzymes have comparable activity to the reconstituted system. Reduction of CYP51 both in the fused enzyme and the reconstituted system is biphasic and consisted of an initial fast phase followed by a slow phase Saccharomyces cerevisiae 4,4-dimethylcholesta-8,14,24-trien-3-ol + NADP+ + H2O
-
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Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
Saccharomyces cerevisiae
1.14.14.154 lanosterol 14-demethylase
-
Saccharomyces cerevisiae