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Literature summary extracted from

  • Yao, J.; Dotson, G.D.
    Kinetic characterization of human phosphopantothenoylcysteine synthetase (2009), Biochim. Biophys. Acta, 1794, 1743-1750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.5 expression in Escherichia coli Homo sapiens
6.3.2.51 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.5 AMP
-
 Homo sapiens
6.3.2.5 ATP
-
 Homo sapiens
6.3.2.5 CMP
-
 Homo sapiens
6.3.2.5 CTP
-
 Homo sapiens
6.3.2.5 GTP
-
 Homo sapiens
6.3.2.5 UTP
-
 Homo sapiens
6.3.2.51 AMP
-
 Homo sapiens
6.3.2.51 ATP
-
 Homo sapiens
6.3.2.51 CMP
-
 Homo sapiens
6.3.2.51 CTP
-
 Homo sapiens
6.3.2.51 GTP
-
 Homo sapiens
6.3.2.51 UTP
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.5 additional information
-
 additional information enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 Homo sapiens
6.3.2.5 0.013
-
 (R)-4'-phosphopantothenate co-substrate ATP, pH 7.6, 37°C Homo sapiens
6.3.2.5 0.014
-
 L-Cys co-substrate ATP, pH 7.6, 37°C Homo sapiens
6.3.2.5 0.016
-
 L-Cys co-substrate CTP, pH 7.6, 37°C Homo sapiens
6.3.2.5 0.057
-
 (R)-4'-phosphopantothenate co-substrate CTP, pH 7.6, 37°C Homo sapiens
6.3.2.5 0.265
-
 CTP pH 7.6, 37°C Homo sapiens
6.3.2.5 0.269
-
 ATP pH 7.6, 37°C Homo sapiens
6.3.2.51 0.013
-
 (R)-4'-phosphopantothenate with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.014
-
 L-cysteine with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.016
-
 L-cysteine with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.057
-
 (R)-4'-phosphopantothenate with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.265
-
 CTP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.269
-
 ATP at pH 7.6 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.51 Mg2+ activity is supported by Mg2+ Homo sapiens
6.3.2.51 Mn2+ activity is supported by Mn2+ Homo sapiens
6.3.2.51 additional information not influenced by Zn2+, Fe2+, Ca2+, and Cd2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.51 34940
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine Homo sapiens
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.5 Homo sapiens
-
-
-
6.3.2.51 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.51 Ni-NTA resin column chromatography, and Mono Q column chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine bi uni uni bi ping pong mechanism. 18O from [carboxyl-18O] phosphopantothenate is incorporated into the AMP or CMP produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate, supporting similar catalytic mechanisms under both CTP and ATP conditions Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.5 ATP + (R)-4'-phosphopantothenate + L-Cys human enzyme can use both ATP and CTP with similar affinity. Catalysis under CTP conditions displays Michaelis-Menten kinetics Homo sapiens (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-Cys human enzyme can use both ATP and CTP with similar affinity. Enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 Homo sapiens (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
 ?
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Homo sapiens AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.51 CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Homo sapiens CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.51 additional information neither GTP nor UTP support enzyme activity with the human enzyme Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.51 homodimer 2 * 34940, electrospray mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.3.2.51 CoaB
-
 Homo sapiens
6.3.2.51 phosphopantothenoylcysteine synthetase
-
 Homo sapiens
6.3.2.51 PPCS
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.5 0.53
-
 CTP pH 7.6, 37°C Homo sapiens
6.3.2.5 0.56
-
 ATP pH 7.6, 37°C Homo sapiens
6.3.2.51 0.53
-
 (R)-4'-phosphopantothenate with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.56
-
 (R)-4'-phosphopantothenate with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.5 0.074
-
 CTP pH 7.6, 37°C Homo sapiens
6.3.2.5 0.372
-
 GTP pH 7.6, 37°C Homo sapiens
6.3.2.5 0.465
-
 ATP pH 7.6, 37°C Homo sapiens
6.3.2.5 0.662
-
 UTP pH 7.6, 37°C Homo sapiens
6.3.2.5 3.7
-
 AMP pH 7.6, 37°C Homo sapiens
6.3.2.5 4.69
-
 CMP pH 7.6, 37°C Homo sapiens
6.3.2.51 0.074
-
 CTP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.372
-
 GTP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.465
-
 ATP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 0.662
-
 UTP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 3.7
-
 AMP at pH 7.6 and 37°C Homo sapiens
6.3.2.51 4.69
-
 CMP at pH 7.6 and 37°C Homo sapiens