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Literature summary extracted from

  • Maravic Vlahovicek, G.; Cubrilo, S.; Tkaczuk, K.L.; Bujnicki, J.M.
    Modeling and experimental analyses reveal a two-domain structure and amino acids important for the activity of aminoglycoside resistance methyltransferase Sgm (2008), Biochim. Biophys. Acta, 1784, 582-590.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.179 expression in Escherichia coli Micromonospora zionensis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.179 D156A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 D158A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 D182A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 E205A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 E267A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 G135A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 K199A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 K54A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 additional information analysis of sequence–function relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis Micromonospora zionensis
2.1.1.179 R108A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 R187S mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 R187S/G212S mutant with strongly reduced activity Micromonospora zionensis
2.1.1.179 R236A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis
2.1.1.179 R433A mutant with drastically increased sensitivity to kanamycin Micromonospora zionensis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.179 Micromonospora zionensis Q7M0R2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.179
-
 Micromonospora zionensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA analysis of sequence–function relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis Micromonospora zionensis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.179 S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
 Micromonospora zionensis S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.179 M7G1405 MTase
-
 Micromonospora zionensis
2.1.1.179 methyltransferase Sgm
-
 Micromonospora zionensis
2.1.1.179 Sgm MTase
-
 Micromonospora zionensis