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Literature summary extracted from

  • Minenko, A.N.; Novikova, L.A.; Luzikov, V.N.; Kovaleva, I.E.
    Import of hybrid forms of CYP11A1 into yeast mitochondria (2008), Biochim. Biophys. Acta, 1780, 1121-1130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.6 hybrid proteins AAC-mCYP11A1, Bcs1p(1–83)-mCYP11A1, Su9(1-116)-mCYP11A1, preAd-mCYP11A1, DLD(1-72)-mCYP11A1 and COXIV-mCYP11A1 expressed in Saccharomyces cerevisiae strain 2805. Escherichia coli strain JM109 transformed with the plasmid pTrc99(A)/Ad-mCYP11A1 synthetic construct

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 additional information all of the addressing sequences, containing transmembrane domains, provide effective insertion of the hybrid proteins AAC-mCYP11A1, Bcs1p(1-83)-mCYP11A1, DLD(1-72)-mCYP11A1 and Su9(1-116)-mCYP11A1 into the mitochondrial inner membrane. preAd-mCYP11A1 hybrid molecules are translocated across the inner membrane and tightly associated with the membrane on its matrix side but not membrane inserted. The mechanism of Ad-mCYP11A1 hybrid topogenesis in Escherichia coli cells differs from that of the topogenesis of its precursor form in yeast mitochondria synthetic construct
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Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 synthetic construct
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 22(R)-hydroxycholesterol + reduced adrenodoxin + O2 mitochondria containing the AAC-mCYP11A1 hybrid have the highest cholesterol side-chain cleavage activity in the reconstituted system. Ad-mCYP11A1 hybrid demonstrates only residual enzyme activity synthetic construct ? + oxidized adrenodoxin + H2O
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.6 CYP11A1
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 synthetic construct
1.14.15.6 cytochrome P450scc
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 synthetic construct

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
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 synthetic construct