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Literature summary extracted from

  • Korotkova, O.G.; Semenova, M.V.; Morozova, V.V.; Zorov, I.N.; Sokolova, L.M.; Bubnova, T.M.; Okunev, O.N.; Sinitsyn, A.P.
    Isolation and properties of fungal beta-glucosidases (2009), Biochemistry, 74, 569-577.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucose competitive inhibition Aspergillus japonicus
3.2.1.21 D-glucose competitive inhibition Talaromyces verruculosus
3.2.1.21 D-glucose competitive inhibition Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.09
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 0.44
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 0.6
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 0.75
-
 cellobiose at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 0.95
-
 cellobiose at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 1.2
-
 cellobiose at pH 5.0 and 40°C Talaromyces verruculosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 116000
-
 SDS-PAGE Talaromyces verruculosus
3.2.1.21 120000
-
 SDS-PAGE Aspergillus japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus japonicus
-
-
-
3.2.1.21 Talaromyces verruculosus
-
-
-
3.2.1.21 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and phenyl-Superose column chromatography Aspergillus japonicus
3.2.1.21 ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and Source 15 isopropyl column chromatography Trichoderma reesei
3.2.1.21 ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and Source 15 isopropyl column chromatography Talaromyces verruculosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 3
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Aspergillus japonicus
3.2.1.21 3
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
3.2.1.21 3 8 using alpha-sophorose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 7
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 9
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 9
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Aspergillus japonicus
3.2.1.21 9
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Trichoderma reesei
3.2.1.21 17
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 18
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 18
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 19
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 20
-
 using cellotriose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 21
-
 using cellobiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 23
-
 using alpha-sophorose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 32
-
 using laminarin as substrate, at pH 5.0 and 50°C Aspergillus japonicus
3.2.1.21 33
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 36
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 39
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Trichoderma reesei
3.2.1.21 45
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 46
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 50
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 52
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
3.2.1.21 54
-
 using cellobiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 54
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 55
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 55
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 56
-
 using cellotriose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 57
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 63
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 69
-
 using cellotriose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 72
-
 using alpha-sophorose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 81
-
 using laminarin as substrate, at pH 5.0 and 50°C Trichoderma reesei
3.2.1.21 83
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 87
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 102
-
 using laminarin as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
3.2.1.21 105
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 124
-
 using cellobiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 147
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 169
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus japonicus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Trichoderma reesei 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O low activity Talaromyces verruculosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 alpha-sophorose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
3.2.1.21 alpha-sophorose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
3.2.1.21 alpha-sophorose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
3.2.1.21 beta-gentiobiose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
3.2.1.21 beta-gentiobiose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
3.2.1.21 beta-gentiobiose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
3.2.1.21 beta-glucan + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.21 beta-glucan + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.21 beta-glucan + H2O low activity Aspergillus japonicus ?
-
 ?
3.2.1.21 cellobiose + H2O low activity, the specific activity towards cellobiose is lower than towards 4-nitrophenyl-beta-D-glucopyranoside Trichoderma reesei 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O low activity, the specific activity towards cellobiose is lower than towards 4-nitrophenyl-beta-D-glucopyranoside Talaromyces verruculosus 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O the specific activity towards cellobiose is higher than towards 4-nitrophenyl-beta-D-glucopyranoside Aspergillus japonicus 2 beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Trichoderma reesei 5 D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Aspergillus japonicus 5 D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Talaromyces verruculosus 5 D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Trichoderma reesei 4 beta-D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Aspergillus japonicus 4 beta-D-glucose
-
 ?
3.2.1.21 cellotetraose + 3 H2O
-
 Talaromyces verruculosus 4 beta-D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Trichoderma reesei 3 D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Aspergillus japonicus 3 D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Talaromyces verruculosus 3 D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Trichoderma reesei 2 beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Aspergillus japonicus 2 beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 Talaromyces verruculosus 2 beta-D-glucose
-
 ?
3.2.1.21 laminarin + H2O low activity Aspergillus japonicus beta-D-glucose + ?
-
 ?
3.2.1.21 laminarin + H2O highest specific activity Trichoderma reesei beta-D-glucose + ?
-
 ?
3.2.1.21 laminarin + H2O highest specific activity Talaromyces verruculosus beta-D-glucose + ?
-
 ?
3.2.1.21 laminaritriose + 2 H2O
-
 Trichoderma reesei 3 beta-D-glucose
-
 ?
3.2.1.21 laminaritriose + 2 H2O
-
 Aspergillus japonicus 3 beta-D-glucose
-
 ?
3.2.1.21 laminaritriose + 2 H2O
-
 Talaromyces verruculosus 3 beta-D-glucose
-
 ?
3.2.1.21 additional information no activity towards carboxymethylcellulose Trichoderma reesei ?
-
 ?
3.2.1.21 additional information no activity towards carboxymethylcellulose Aspergillus japonicus ?
-
 ?
3.2.1.21 additional information no activity towards carboxymethylcellulose Talaromyces verruculosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-D-glucoside glucohydrolase
-
 Trichoderma reesei
3.2.1.21 beta-D-glucoside glucohydrolase
-
 Aspergillus japonicus
3.2.1.21 beta-D-glucoside glucohydrolase
-
 Talaromyces verruculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
 at pH 5.0 Talaromyces verruculosus
3.2.1.21 65
-
 at pH 5.0 Aspergillus japonicus
3.2.1.21 70
-
 at pH 5.0 Trichoderma reesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 40 80
-
 Talaromyces verruculosus
3.2.1.21 48 72
-
 Aspergillus japonicus
3.2.1.21 50 80
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme loses 70-85% of its activity, after 1 h of incubation at 50°C the enzyme retains 20% of its activity Talaromyces verruculosus
3.2.1.21 30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme loses 70-85% of its activity, after 1 h of incubation at 50°C the enzyme retains 43% of its activity Trichoderma reesei
3.2.1.21 30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme retains 50% of its activity, after 1 h of incubation at 50°C the enzyme retains 57% of its activity Aspergillus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 42
-
 cellobiose at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 89
-
 cellobiose at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 118
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 160
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 259
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Aspergillus japonicus
3.2.1.21 350
-
 cellobiose at pH 5.0 and 40°C Aspergillus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5 5 at 40°C Aspergillus japonicus
3.2.1.21 5
-
 at 40°C Talaromyces verruculosus
3.2.1.21 5.5
-
 at 40°C Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.51
-
 D-glucose at pH 5.0 and 40°C Trichoderma reesei
3.2.1.21 0.93
-
 D-glucose at pH 5.0 and 40°C Talaromyces verruculosus
3.2.1.21 2.73
-
 D-glucose at pH 5.0 and 40°C Aspergillus japonicus