Literature summary extracted from
Robins, L.I.; Williams, A.H.; Raetz, C.R.
Structural basis for the sugar nucleotide and acyl-chain selectivity of Leptospira interrogans LpxA (2009), Biochemistry, 48, 6191-6201.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.129 |
expressed in Escherichia coli |
Leptospira interrogans |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.3.1.129 |
crystal structures of free LiLpxA and its complexes with its product UDP-3-N-((R)-3-hydroxylauroyl)-GlcNAc3N and with its substrate (R)-3-hydroxylauroyl-methylphosphopantetheine are presented. The selectivity of LiLpxA for UDPGlcNAc3N may be explained by the orientation of the backbone carbonyl group of Q68, which differs by 82° from the corresponding Q73 carbonyl group in Escherichia coli LpxA. This arrangement provides an extra hydrogen-bond acceptor for the 3-NH2 group of UDP-GlcNAc3N in LiLpxA. The R-3-hydroxylauroyl selectivity of LiLpxA is explained by the position of the K171 side chain, which limits the length of the acyl-chain-binding groove. H120 functions as a catalytic base |
Leptospira interrogans |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.3.1.129 |
0.004 |
- |
3R-3-hydroxylauroyl-methylphosphopantetheine |
- |
Leptospira interrogans |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.129 |
Leptospira interrogans |
Q8EZA6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.129 |
ion-exchange-chromatography and gel filtration |
Leptospira interrogans |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.3.1.129 |
3 |
- |
using synthetic (3R)-3-hydroxylauroyl-methylphosphopantetheine + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose as a substrate the specific activity is comparable to substrates (3R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
Leptospira interrogans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.129 |
(3R)-3-hydroxylauroyl-methylphosphopantetheine + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
synthetic (3R)-3-hydroxylauroyl-methylphosphopantetheine is an excellent alternative donor substrate for LiLpxA |
Leptospira interrogans |
methylphosphopantetheine + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
- |
? |
|
2.3.1.129 |
(R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
LiLpxA is highly selective for (R)-3-hydroxylauroyl-[acyl-carrier protein] and UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
Leptospira interrogans |
[acyl-carrier protein] + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.1.129 |
homotrimer |
in aqueous solution, shown by gel filtration |
Leptospira interrogans |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.129 |
LiLpxA |
- |
Leptospira interrogans |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.3.1.129 |
30 |
- |
assay at |
Leptospira interrogans |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.3.1.129 |
8 |
- |
assay at |
Leptospira interrogans |