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Literature summary extracted from

  • Go, M.K.; Amyes, T.L.; Richard, J.P.
    Hydron transfer catalyzed by triosephosphate isomerase. Products of the direct and phosphite-activated isomerization of [1-(13)C]-glycolaldehyde in D2O (2009), Biochemistry, 48, 5769-5778.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expression of the wild-type enzyme in TIM-deficient Escherichia coli strain DF502 Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate Gallus gallus
-
 Glycerone phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 recombinant wild-type enzyme from TIM-deficient Escherichia coli strain DF502 by anion exchange chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.1 skeletal muscle
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.1 additional information
-
 quantitative TIM-catalyzed isomerization of D-glyceraldehyde 3-phosphate to form glycerone phosphate is coupled to the oxidation of NADH using glycerol 3-phosphate dehydrogenase Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
 Gallus gallus Glycerone phosphate
-
 r
5.3.1.1 D-Glyceraldehyde 3-phosphate stereospecific reaction via an enzyme-bound enediol(ate) intermediate, proton transfer from D-glyceraldehyde 3-phosphate to the carboxylate side chain of TIM Glu165, irreversible labeling with deuterium , overview Gallus gallus Glycerone phosphate
-
 r
5.3.1.1 glycolaldehyde TIM-catalyzed reactions of glycolaldehyde are activated by phosphite dianion, kinetics, overview Gallus gallus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TIM
-
 Gallus gallus
5.3.1.1 Triosephosphate isomerase
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.1 25
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.1 7
-
 assay at Gallus gallus