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Literature summary extracted from

  • Yao, J.; Patrone, J.D.; Dotson, G.D.
    Characterization and kinetics of phosphopantothenoylcysteine synthetase from Enterococcus faecalis (2009), Biochemistry, 48, 2799-2806.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.33 synthesis synthesis of (carboxyl-18O)phosphopantothenate Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.5 expression in Escherichia coli Enterococcus faecalis
6.3.2.51 expressed in Escherichia coli BL21 AI cells Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.5 CMP competitive to CTP, mixed type to (R)-4'-phosphopantothenate, uncompetitive to L-Cys Enterococcus faecalis
6.3.2.5 L-Cys
-
 Enterococcus faecalis
6.3.2.51 CTP
-
 Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.5 0.017
-
 (R)-4'-phosphopantothenate pH 7.6, 37°C Enterococcus faecalis
6.3.2.5 0.086
-
 L-Cys pH 7.6, 37°C Enterococcus faecalis
6.3.2.5 0.156
-
 CTP pH 7.6, 37°C Enterococcus faecalis
6.3.2.51 0.017
-
 (R)-4'-phosphopantothenate at pH 7.6 and 37°C Enterococcus faecalis
6.3.2.51 0.086
-
 L-cysteine at pH 7.6 and 37°C Enterococcus faecalis
6.3.2.51 0.156
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.5 28415
-
 2 * 28415, ESI-MS Enterococcus faecalis
6.3.2.5 60000
-
 gel filtration Enterococcus faecalis
6.3.2.51 28000
-
 2 * 28000, SDS-PAGE Enterococcus faecalis
6.3.2.51 28415
-
-
 Enterococcus faecalis
6.3.2.51 60000
-
 gel filtration Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine Enterococcus faecalis
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Enterococcus faecalis Q839J7
-
-
6.3.2.5 Enterococcus faecalis
-
-
-
6.3.2.51 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.5 recombinant enzyme Enterococcus faecalis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine bi uni uni bi ping pong mechanism, with the nucleotide CTP binding first and CMP released last Enterococcus faecalis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 pantothenate + ATP
-
 Enterococcus faecalis phosphopantothenate + ADP
-
 ?
6.3.2.5 ATP + (R)-4'-phosphopantothenate + L-Cys 8% of the activity with CTP Enterococcus faecalis (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate
-
 ?
6.3.2.5 CTP + (R)-4'-phosphopantothenate + L-Cys
-
 Enterococcus faecalis (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
 ?
6.3.2.51 ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Enterococcus faecalis AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
6.3.2.51 CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Enterococcus faecalis CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.5 dimer 2 * 28415, ESI-MS Enterococcus faecalis
6.3.2.51 homodimer 2 * 28000, SDS-PAGE Enterococcus faecalis
6.3.2.51 homodimer 2 * 28415, electrospray ionization mass spectroemtry Enterococcus faecalis
6.3.2.51 homodimer 2 * 28525, calculated from amino acid sequence Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
 Enterococcus faecalis
2.7.1.33 pantothenate kinase
-
 Enterococcus faecalis
6.3.2.51 CoaB
-
 Enterococcus faecalis
6.3.2.51 phosphopantothenoylcysteine synthetase
-
 Enterococcus faecalis
6.3.2.51 PPCS
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.5 2.9
-
 CTP pH 7.6, 37°C Enterococcus faecalis
6.3.2.51 2.9
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis
6.3.2.51 2.9
-
 L-cysteine at pH 7.6 and 37°C Enterococcus faecalis
6.3.2.51 2.9
-
 (R)-4'-phosphopantothenate at pH 7.6 and 37°C Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.5 0.766
-
 L-Cys substrate CTP, pH 7.6, 37°C Enterococcus faecalis
6.3.2.51 0.652
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis