EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.3.13 | medicine | alpha-isopropylmalate synthase is a target for the design of new antitubercular therapeutics | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | into the vector pET28a for expression in Escherichia coli BL21DE3 pLysS cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.13 | E218A | mutant, substitution at phylogenetically conserved active site residue near the interface of the catalytic and linker domains | Mycobacterium tuberculosis |
2.3.3.13 | H379A | mutant, substitution at phylogenetically conserved active site residue near the interface of the catalytic and linker domains | Mycobacterium tuberculosis |
2.3.3.13 | Y169F | mutant, substitution at phylogenetically conserved active site residue near the interface of the catalytic and linker domains | Mycobacterium tuberculosis |
2.3.3.13 | Y410F | mutant, insensitive to L-leucine inhibition | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | L-leucine | feedback inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.0005 | - |
alpha-ketoisovalerate | mutant Y410F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0011 | - |
acetyl-CoA | mutant Y410F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0016 | - |
alpha-ketoisovalerate | mutant E218A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0016 | - |
alpha-ketoisovalerate | mutant H379A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0023 | - |
acetyl-CoA | mutant H379A | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0071 | - |
alpha-ketoisovalerate | wild-type | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0095 | - |
acetyl-CoA | mutant E218A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.0134 | - |
alpha-ketoisovalerate | mutant Y169F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.019 | - |
alpha-ketoisovalerate | mutant H379A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.024 | - |
acetyl-CoA | wild-type | Mycobacterium tuberculosis | |
2.3.3.13 | 0.039 | - |
alpha-ketoisovalerate | mutant E218A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.04 | - |
acetyl-CoA | mutant Y169F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.697 | - |
acetyl-CoA | mutant E218A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis H37Rv | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Mycobacterium tuberculosis | P9WQB3 | - |
- |
2.3.3.13 | Mycobacterium tuberculosis H37Rv | P9WQB3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | on a nickel-NTA, a Mono-Q and a Phenyl Sepharose column | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis H37Rv | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | alpha-ketoisovalerate + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis | ? + CoA | - |
? | |
2.3.3.13 | alpha-ketoisovalerate + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis H37Rv | ? + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
2.3.3.13 | MtIPMS | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 25 | - |
activity assay | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.022 | - |
alpha-ketoisovalerate | mutant E218A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.037 | - |
alpha-ketoisovalerate | mutant E218A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.058 | - |
acetyl-CoA | mutant E218A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.075 | - |
alpha-ketoisovalerate | mutant Y410F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.093 | - |
acetyl-CoA | mutant E218A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis | |
2.3.3.13 | 0.093 | - |
acetyl-CoA | mutant Y410F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.7 | - |
alpha-ketoisovalerate | mutant H379A, enzyme displays substrate activation, unactivated form | Mycobacterium tuberculosis | |
2.3.3.13 | 1.483 | - |
alpha-ketoisovalerate | mutant H379A, enzyme displays substrate activation, activated form | Mycobacterium tuberculosis | |
2.3.3.13 | 2.53 | - |
alpha-ketoisovalerate | wild-type | Mycobacterium tuberculosis | |
2.3.3.13 | 2.55 | - |
acetyl-CoA | mutant H379A | Mycobacterium tuberculosis | |
2.3.3.13 | 2.717 | - |
acetyl-CoA | wild-type | Mycobacterium tuberculosis | |
2.3.3.13 | 4.75 | - |
alpha-ketoisovalerate | mutant Y169F | Mycobacterium tuberculosis | |
2.3.3.13 | 5.667 | - |
acetyl-CoA | mutant Y169F | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 7.5 | - |
activity assay | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | acetyl-CoA | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.012 | - |
L-leucine | wild-type | Mycobacterium tuberculosis | |
2.3.3.13 | 0.018 | - |
L-leucine | mutant Y169F | Mycobacterium tuberculosis | |
2.3.3.13 | 0.019 | - |
L-leucine | mutant H379A | Mycobacterium tuberculosis | |
2.3.3.13 | 0.139 | - |
L-leucine | mutant E218A | Mycobacterium tuberculosis |