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Literature summary extracted from

  • Seifert, F.; Schulz, K.; Koch, B.; Manhart, S.; Demuth, H.U.; Schilling, S.
    Glutaminyl cyclases display significant catalytic proficiency for glutamyl substrates (2009), Biochemistry, 48, 11831-11833.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5 expressed in Pichia pastoris Mus musculus
2.3.2.5 expressed in Pichia pastoris Homo sapiens
2.3.2.5 expressed in Pichia pastoris Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.5 EFRH-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Homo sapiens
2.3.2.5 EFRH-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Mus musculus
2.3.2.5 EFRHHDSGYE-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Homo sapiens
2.3.2.5 EFRHHDSGYE-NH2 competitive inhibitor with L-glutaminyl-7-amido-4-methylcumarin as substrate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.5 4.6
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
2.3.2.5 6
-
 EFRH-NH2 at pH 6.5, 10times higher compared to KM-value of Gln substrate Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.5 Zn2+
-
 Mus musculus
2.3.2.5 Zn2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Carica papaya
-
-
-
2.3.2.5 Homo sapiens
-
-
-
2.3.2.5 Mus musculus
-
-
-
2.3.2.5 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5
-
 Mus musculus
2.3.2.5
-
 Homo sapiens
2.3.2.5
-
 Solanum tuberosum
2.3.2.5
-
 Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 EFRH-NH2
-
 Mus musculus pEFRH-NH2 + H2O
-
 ?
2.3.2.5 EFRH-NH2
-
 Homo sapiens pEFRH-NH2 + H2O
-
 ?
2.3.2.5 EFRH-NH2
-
 Solanum tuberosum pEFRH-NH2 + H2O
-
 ?
2.3.2.5 EFRH-NH2
-
 Carica papaya pEFRH-NH2 + H2O
-
 ?
2.3.2.5 EFRHHDSGYE-NH2
-
 Mus musculus pEFRHHDSGYE-NH2 + H2O
-
 ?
2.3.2.5 EFRHHDSGYE-NH2
-
 Homo sapiens pEFRHHDSGYE-NH2 + H2O
-
 ?
2.3.2.5 EFRHHDSGYE-NH2
-
 Solanum tuberosum pEFRHHDSGYE-NH2 + H2O
-
 ?
2.3.2.5 EFRHHDSGYE-NH2
-
 Carica papaya pEFRHHDSGYE-NH2 + H2O
-
 ?
2.3.2.5 L-glutaminyl-7-amido-4-methylcoumarin
-
 Homo sapiens pGlu-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 QFRH-NH2
-
 Mus musculus pEFRH-NH2 + NH3
-
 ?
2.3.2.5 QFRH-NH2
-
 Homo sapiens pEFRH-NH2 + NH3
-
 ?
2.3.2.5 QFRH-NH2
-
 Solanum tuberosum pEFRH-NH2 + NH3
-
 ?
2.3.2.5 QFRH-NH2
-
 Carica papaya pEFRH-NH2 + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 glutaminyl cyclase
-
 Mus musculus
2.3.2.5 glutaminyl cyclase
-
 Homo sapiens
2.3.2.5 glutaminyl cyclase
-
 Solanum tuberosum
2.3.2.5 glutaminyl cyclase
-
 Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.5 0.00022
-
 EFRH-NH2 at pH 6.5, 150000fold lower compared to Gln substrate Homo sapiens
2.3.2.5 0.00042
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
2.3.2.5 30
-
 QFRH-NH2 at pH 6.5 Homo sapiens
2.3.2.5 40
-
 QFRH-NH2 at pH 6.5 Mus musculus
2.3.2.5 42
-
 QFRH-NH2 at pH 6.5 Carica papaya
2.3.2.5 56
-
 QFRH-NH2 at pH 6.5 Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.5 6.8
-
 EFRH-NH2 as substrate Homo sapiens
2.3.2.5 7.5 7.8 Gln-peptide as substrate Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.5 3.25
-
 EFRH-NH2 at pH 8.0 Homo sapiens
2.3.2.5 4.69
-
 EFRHHDSGYE-NH2 at pH 8.0 Homo sapiens
2.3.2.5 6.1
-
 EFRHHDSGYE-NH2 at pH 8.0 Mus musculus
2.3.2.5 8.32
-
 EFRHHDSGYE-NH2 at pH 6.0 Homo sapiens
2.3.2.5 8.33
-
 EFRH-NH2 at pH 8.0 Mus musculus
2.3.2.5 10.4
-
 EFRHHDSGYE-NH2 at pH 6.0 Mus musculus
2.3.2.5 24.64
-
 EFRH-NH2 at pH 6.0 Homo sapiens
2.3.2.5 35.75
-
 EFRH-NH2 at pH 6.0 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.5 0.000019
-
 EFRH-NH2 at pH 6.5 Mus musculus
2.3.2.5 0.000037
-
 EFRH-NH2 at pH 6.5 Homo sapiens
2.3.2.5 0.000073
-
 EFRHHDSGYE-NH2 at low substrate concentrations Homo sapiens
2.3.2.5 0.00009
-
 EFRHHDSGYE-NH2
-
 Homo sapiens
2.3.2.5 0.00017
-
 EFRH-NH2 at pH 6.5 Solanum tuberosum
2.3.2.5 0.00074
-
 EFRH-NH2 at pH 6.5 Carica papaya
2.3.2.5 98.9
-
 QFRH-NH2 at pH 6.5 Solanum tuberosum
2.3.2.5 100
-
 QFRH-NH2 at pH 6.5 Homo sapiens
2.3.2.5 135
-
 QFRH-NH2 at pH 6.5 Mus musculus
2.3.2.5 145
-
 QFRH-NH2 at pH 6.5 Carica papaya