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Literature summary extracted from

  • di Luccio, E.; Wilson, D.K.
    Comprehensive X-ray structural studies of the quinolinate phosphoribosyl transferase (BNA6) from Saccharomyces cerevisiae (2008), Biochemistry, 47, 4039-4050.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.19 into plasmid pTYB12 and expressed in Escherichia coli BL21 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.19 apo, quinolinate-bound, 5-phospho-alpha-D-ribose 1-diphosphate-bound, and phthalate-bound forms. Crystallized at room temperature by the hanging drop vapor diffusion method. Both apo and holo crystals belong to space group R32 (alpha = 90°, beta = 90°, and gamma = 120°). One molecule per asymmetric unit except in QAPRTase holophthalate crystals where two molecules are found. The unit cell dimensions are: a = b = 154.9 A and c = 68.9 A (apo), a = b = 154.8 A and c = 68.6 A (holoquinolinate), a = b = 154.9 A and c = 70.6 A (holo-5-phospho-alpha-D-ribose 1-diphosphate), a = b = 155.5 A and c = 121.1 A (holophthalate), a = b = 154.7 A and c = 69.3 A (holophthalate-5-phospho-alpha-D-ribose 1-diphosphate) Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.19 Phthalate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.19 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.19 191000
-
 6 * 191000, dynamic light scattering analysis Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.19 Saccharomyces cerevisiae P43619
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.19 by anion exchange chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.19 quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae nicotinic acid mononucleotide + diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.19 hexamer 6 * 191000, dynamic light scattering analysis Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.4.2.19 BNA6
-
 Saccharomyces cerevisiae
2.4.2.19 QAPRTase
-
 Saccharomyces cerevisiae
2.4.2.19 quinolinate phosphoribosyl transferase
-
 Saccharomyces cerevisiae