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Literature summary extracted from

  • Gralla, J.D.; Huo, Y.X.
    Remodeling and activation of Escherichia coli RNA polymerase by osmolytes (2008), Biochemistry, 47, 13189-13196.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.6 glutamate glutamate remodels the sigma38 transcription complex for activation. Accumulation of the simple signaling molecule glutamate can reprogram RNA polymerase in vitro without the need for specific protein receptors. During osmotic activation, glutamate appears to act as a Hofmeister series osmolyte to facilitate promoter escape. Escape is accompanied by a remodeling of the key interaction between the sigma38 stress protein and the beta-flap of the bacterial core RNA polymerase. This activation event contrasts with the established mechanism of inhibition in which glutamate, by virtue of its electrostatic properties, helps to inhibit binding to ribosomal promoters after osmotic shock Escherichia coli
2.7.7.6 potassium acetate activates Escherichia coli
2.7.7.6 potassium aspartate activates Escherichia coli
2.7.7.6 potassium chloride activates Escherichia coli
2.7.7.6 potassium glutamate activates highly, role of potassium ion in the activation of osmotic transcription Escherichia coli
2.7.7.6 potassium nitrate activates Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Escherichia coli regulation by anions, overview diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn regulation by anions, overview Escherichia coli diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is supercoiled DNA Escherichia coli diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase
-
 Escherichia coli
2.7.7.6 sigma38 RNA polymerase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
 assay at Escherichia coli