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Literature summary extracted from
- Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase (1984), Biochemistry, 23, 445-453.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.5.1 | cytoplasmic membrane | inner surface of the cytoplasmic membrane and is coupled to the Escherichia coli aerobic respiratory chain | Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.5.1 | pyruvate + ubiquinone-8 | Escherichia coli | the role of quinones in the pyruvate oxidase system is investigated in this paper | acetate + CO2 + ubiquinol-8 | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.5.1 | Escherichia coli | - |
- |
- |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.2.5.1 | reconstitution of a minimal respiratory chain consisting of pyruvate oxidase and cytochrome d terminal oxidase plus ubiquinone8 incorporated in phospholipid vesicles. The catalytic velocity of the reconstituted liposome system is about 30% of that observed when the flavoprotein is reconstituted with Escherichia coli membranes | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.5.1 | pyruvate + ubiquinone-8 | the role of quinones in the pyruvate oxidase system is investigated in this paper | Escherichia coli | acetate + CO2 + ubiquinol-8 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.5.1 | ubiquinione-dependent pyruvate oxidase | - |
Escherichia coli |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.2.5.1 | physiological function | electron transport from pyruvate to oxygen in the liposome system generates a trans-membrane potential of at least 180 mV, negative inside, which is sensitive to the uncouplers carbonyl cyanide p-(trichloromethoxy)phenylhydrazone and valinomycin. A transmembrane potential is also generated by the oxidation of ubiquinol 1 by the terminal oxidase in the absence of the flavoprotein. The pyruvate oxidase can directly reduce ubiquinone 8 within the phospholipid bilayer, menaquinone 8 will not effectively substitute for ubiquinone 8 in this electron-transfer chain, and the cytochrome d terminal oxidase functions as a ubiquinol 8 oxidase and serves as a coupling site in the Escherichia coli aerobic respiratory chain | Escherichia coli |
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Release 2023.1 (January 2023)
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