EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.82 | medicine | ethanolamine kinase could be a target for antimalerial therapy | Plasmodium falciparum 3D7 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.32 | His-tag, expressed in Escherichia coli BL21(DE3)-pRIL | Plasmodium falciparum 3D7 |
2.7.1.82 | expression in Escherichia coli | Plasmodium falciparum |
2.7.1.82 | into the vector pET24b for expression in Escherichia coli BL21DE3-pRIL cells | Plasmodium falciparum 3D7 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.32 | 2-amino-1-butanol | - |
Plasmodium falciparum 3D7 | |
2.7.1.32 | hemicholinium-3 | - |
Plasmodium falciparum 3D7 | |
2.7.1.32 | SAR97276 | - |
Plasmodium falciparum 3D7 | |
2.7.1.82 | 2-amino-1-butanol | analogue of ethanolamine, selective inhibition | Plasmodium falciparum | |
2.7.1.82 | 2-amino-1-butanol | - |
Plasmodium falciparum 3D7 | |
2.7.1.82 | 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | SAR97276 | Plasmodium falciparum 3D7 | |
2.7.1.82 | hemicholinium-3 | - |
Plasmodium falciparum | |
2.7.1.82 | SAR97276 | bis-thiazolium compound designed as a choline analogue, affects ethanolamine kinase and choline kinase activities similarly. Inhibition is competitive and correlates with the impairment of cellular phosphatidylcholine biosynthesis | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | additional information | - |
ethanolamine | above 500 mM | Plasmodium falciparum 3D7 | |
2.7.1.32 | 0.1353 | - |
choline | recombinant protein | Plasmodium falciparum 3D7 | |
2.7.1.82 | 0.458 | - |
ethanolamine | pH 8.0, 37°C | Plasmodium falciparum | |
2.7.1.82 | 0.4757 | - |
ethanolamine | - |
Plasmodium falciparum 3D7 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.82 | cytoplasm | - |
Plasmodium falciparum 3D7 | 5737 | - |
2.7.1.82 | cytoplasm | of Plasmodium falciparum | Plasmodium falciparum | 5737 | - |
2.7.1.82 | additional information | protein expression levels increase during erythrocytic development | Plasmodium falciparum | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | 52000 | - |
1 * 52000, gel filtration | Plasmodium falciparum 3D7 |
2.7.1.82 | 48000 | - |
determined by SDS-PAGE and Western Blot analysis | Plasmodium falciparum 3D7 |
2.7.1.82 | 109000 | - |
determined by gel filtration | Plasmodium falciparum 3D7 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.82 | ATP + ethanolamine | Plasmodium falciparum 3D7 | - |
ADP + O-phosphoethanolamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.32 | Plasmodium falciparum 3D7 | Q8IM71 | - |
- |
2.7.1.82 | Plasmodium falciparum | Q8IIB7 | 3D7 | - |
2.7.1.82 | Plasmodium falciparum 3D7 | Q8IIB7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.32 | - |
Plasmodium falciparum 3D7 |
2.7.1.82 | using a HisTrap chelating and a gel filtration column | Plasmodium falciparum 3D7 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.32 | additional information | - |
62.2 micromol/min/mg for ethanolamine | Plasmodium falciparum 3D7 |
2.7.1.32 | 10.8 | - |
recombinant protein | Plasmodium falciparum 3D7 |
2.7.1.82 | 1.3 | - |
pH 8.0, 37°C | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.32 | choline + ATP | - |
Plasmodium falciparum 3D7 | O-phosphocholine + ADP | - |
? | |
2.7.1.82 | ATP + ethanolamine | - |
Plasmodium falciparum 3D7 | ADP + O-phosphoethanolamine | - |
? | |
2.7.1.82 | ATP + ethanolamine | - |
Plasmodium falciparum | ADP + O-phosphoethanolamine | - |
? | |
2.7.1.82 | additional information | no substrate: choline | Plasmodium falciparum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.32 | monomer | 1 * 52000, gel filtration | Plasmodium falciparum 3D7 |
2.7.1.82 | homodimer | - |
Plasmodium falciparum 3D7 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.32 | choline kinase | - |
Plasmodium falciparum 3D7 |
2.7.1.82 | ethanolamine kinase | - |
Plasmodium falciparum 3D7 |
2.7.1.82 | PfEK | - |
Plasmodium falciparum 3D7 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.82 | 37 | - |
activity assay | Plasmodium falciparum 3D7 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.82 | 8 | - |
activity assay | Plasmodium falciparum 3D7 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.82 | ATP | - |
Plasmodium falciparum 3D7 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.9 | - |
SAR97276 | calculated from Dixon analysis | Plasmodium falciparum 3D7 | |
2.7.1.32 | 1.03 | - |
SAR97276 | calculated from Lineweaver-Burk analysis | Plasmodium falciparum 3D7 | |
2.7.1.82 | 0.73 | - |
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | - |
Plasmodium falciparum 3D7 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.1.32 | 0.22 | - |
endogenous enzyme | Plasmodium falciparum 3D7 | hemicholinium-3 | |
2.7.1.32 | 0.52 | - |
endogenous enzyme | Plasmodium falciparum 3D7 | SAR97276 | |
2.7.1.32 | 0.58 | - |
recombinant enzyme | Plasmodium falciparum 3D7 | hemicholinium-3 | |
2.7.1.32 | 1.12 | - |
recombinant enzyme | Plasmodium falciparum 3D7 | SAR97276 | |
2.7.1.32 | 3.2 | - |
endogenous enzyme | Plasmodium falciparum 3D7 | 2-amino-1-butanol | |
2.7.1.82 | 0.46 | - |
pH 8.0, 37°C | Plasmodium falciparum | hemicholinium-3 | |
2.7.1.82 | 0.46 | - |
Plasmodium falciparum-endogenous ethanolamine kinase | Plasmodium falciparum 3D7 | 2-amino-1-butanol | |
2.7.1.82 | 0.61 | - |
recombinant protein | Plasmodium falciparum 3D7 | 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | |
2.7.1.82 | 0.79 | - |
pH 8.0, 37°C | Plasmodium falciparum | SAR97276 | |
2.7.1.82 | 0.79 | - |
Plasmodium falciparum-endogenous ethanolamine kinase | Plasmodium falciparum 3D7 | 3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium] | |
2.7.1.82 | 1.36 | - |
pH 8.0, 37°C | Plasmodium falciparum | 2-amino-1-butanol | |
2.7.1.82 | 1.36 | - |
recombinant protein | Plasmodium falciparum 3D7 | 2-amino-1-butanol |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.7.1.82 | Plasmodium falciparum 3D7 | expression levels increases during erythrocytic development | up |