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Literature summary extracted from

  • Kotake, T.; Takata, R.; Verma, R.; Takaba, M.; Yamaguchi, D.; Orita, T.; Kaneko, S.; Matsuoka, K.; Koyama, T.; Reiter, W.D.; Tsumuraya, Y.
    Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants (2009), Biochem. J., 424, 169-177.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 gene UGE1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the enzyme fused to thioredoxin and His6 in Escherichia coli strain BL21(DE3) Pisum sativum
5.1.3.2 recombinant protein is expressed in Escherichia coli Pisum sativum
5.1.3.2 recombinant protein is expressed in Escherichia coli with C-terminal His-tag Arabidopsis thaliana
5.1.3.5 recombinant protein AtUGE1-5 is expressed in Escherichia coli with C-terminal His-tag Arabidopsis thaliana
5.1.3.5 recombinant protein is expressed in Escherichia coli Pisum sativum
5.1.3.5 recombinant protein is expressed in Escherichia coli with C-terminal His-tag Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.2 Hg2+ complete inhibition, recombinan t enzyme Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 additional information
-
 additional information kinetic analysis Pisum sativum
5.1.3.2 0.057
-
 UDP-D-galactose recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase Arabidopsis thaliana
5.1.3.2 0.068
-
 UDP-D-galactose recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase Arabidopsis thaliana
5.1.3.2 0.087
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase Arabidopsis thaliana
5.1.3.2 0.095
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDPGlc dehydrogenase Arabidopsis thaliana
5.1.3.2 0.15
-
 UDP-D-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 0.15
-
 UDP-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 0.15
-
 UDP-D-galactose recombinant enzyme UGE5, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.2 0.16
-
 UDP-L-arabinose pH 8.6, 25°C Pisum sativum
5.1.3.2 0.29
-
 UDP-galactose pH 8.6, 25°C Pisum sativum
5.1.3.2 0.29
-
 UDP-D-galactose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
5.1.3.2 0.31
-
 UDP-glucose pH 8.6, 25°C Pisum sativum
5.1.3.2 0.31
-
 UDP-D-glucose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
5.1.3.5 0.015
-
 UDP-D-xylose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
5.1.3.5 0.016
-
 UDP-L-arabinose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
5.1.3.5 0.16
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 0.32
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 0.34
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE2, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 0.42
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE4, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 0.43
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.3.2 cytosol
-
 Arabidopsis thaliana 5829
-
5.1.3.2 cytosol
-
 Pisum sativum 5829
-
5.1.3.2 cytosol the bifunctional UDP-Glc 4-epimerase/UDP-Xyl 4-epimerase, encoded by UGE1, in the cytosol is distinct from the UDP-Xyl 4-epimerase in the Golgi apparatus Pisum sativum 5829
-
5.1.3.5 cytosol
-
 Arabidopsis thaliana 5829
-
5.1.3.5 cytosol
-
 Pisum sativum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.2 Al3+ enzyme activity is 28.6 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Ba2+ enzyme activity is 54.6 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Ca2+ enzyme activity is 47.8 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Co2+ enzyme activity is 44.7 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Cu2+ enzyme activity is 21.8 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Fe3+ enzyme activity is 20.3 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Hg2+ 1 mM Hg2+ abolishes enzyme function entirely Pisum sativum
5.1.3.2 Li+ enzyme activity is 43.7 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Mg2+ enzyme activity is 51.5 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 Mn2+ enzyme activity is 43.2 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.2 additional information no metal ion requirement of the recombinant protein for either UDP-Glc 4-epimerase or UDP-Xyl 4-epimerase activity Pisum sativum
5.1.3.2 Zn2+ enzyme activity is 17.2 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Al3+ activity of enzyme towards UDP-xylose is 15.5 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Ba2+ activity of enzyme towards UDP-xylose is 23.0 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Ca2+ activity of enzyme towards UDP-xylose is 20.2 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Co2+ activity of enzyme towards UDP-xylose is 18.6 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Cu2+ activity of enzyme towards UDP-xylose is 13.9 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Fe3+ activity of enzyme towards UDP-xylose is 12.1 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Hg2+ 1 mM Hg2+ abolishes enzyme function entirely Pisum sativum
5.1.3.5 Li+ activity of enzyme towards UDP-xylose is 18.1 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Mg2+ activity of enzyme towards UDP-xylose is 21.3 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Mn2+ activity of enzyme towards UDP-xylose is 15.5 U/mg protein at a metal concentration of 1 mM Pisum sativum
5.1.3.5 Zn2+ activity of enzyme towards UDP-xylose is 10.2 U/mg protein at a metal concentration of 1 mM Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 39000
-
 native Pisum sativum enzyme determined by SDS-PAGE Pisum sativum
5.1.3.2 41000
-
 recombinant enzyme after purification and thrombin digestion Pisum sativum
5.1.3.2 56000
-
 recombinant enzyme after purification on a chelating column Pisum sativum
5.1.3.5 39000
-
 native Pisum sativum enzyme determined by SDS-PAGE Pisum sativum
5.1.3.5 41000
-
 recombinant enzyme after purification and thrombin digestion Pisum sativum
5.1.3.5 56000
-
 recombinant enzyme after purification on a chelating column Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 UDP-alpha-D-glucose Pisum sativum
-
 UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-D-glucose Arabidopsis thaliana
-
 UDP-D-galactose
-
 r
5.1.3.2 UDP-D-glucose Pisum sativum
-
 UDP-D-galactose
-
 r
5.1.3.2 UDP-D-xylose Pisum sativum
-
 UDP-L-arabinose
-
 r
5.1.3.2 UDP-galactose Pisum sativum
-
 UDP-glucose
-
 r
5.1.3.5 UDP-D-xylose Arabidopsis thaliana
-
 UDP-L-arabinose
-
 r
5.1.3.5 UDP-D-xylose Pisum sativum
-
 UDP-L-arabinose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Arabidopsis thaliana Q42605
-
-
5.1.3.2 Arabidopsis thaliana Q8LDN8
-
-
5.1.3.2 Arabidopsis thaliana Q9C7W7
-
-
5.1.3.2 Arabidopsis thaliana Q9SN58
-
-
5.1.3.2 Arabidopsis thaliana Q9T0A7
-
-
5.1.3.2 Pisum sativum B0M3E8
-
-
5.1.3.2 Pisum sativum B0M3E8 gene UGE1
-
5.1.3.5 Arabidopsis thaliana Q42605
-
-
5.1.3.5 Arabidopsis thaliana Q8LDN8
-
-
5.1.3.5 Arabidopsis thaliana Q9C7W7
-
-
5.1.3.5 Arabidopsis thaliana Q9SN58
-
-
5.1.3.5 Arabidopsis thaliana Q9T0A7
-
-
5.1.3.5 Pisum sativum B0M3E8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2 purification of the native enzyme from pea sprouts by ion exchange chromatography and gel filtration, further purification on a hydroxyapatite column, purification of recombinant enzymes by ion exchange chromatography Pisum sativum
5.1.3.2 purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography Arabidopsis thaliana
5.1.3.2 purification of the recombinant enzyme by Ni-NTA affinity chromatography Arabidopsis thaliana
5.1.3.2 recombinant fusion enzyme 2.33fold from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, native UGE1 334fold from sprouts by ammonium sulfate fractionation and a chromatographic procedure Pisum sativum
5.1.3.5 purification of the native enzyme from pea sprouts by ion exchange chromatography and gel filtration, further purification on a hydroxyapatite column, purification of recombinant enzymes by ion exchange chromatography Pisum sativum
5.1.3.5 purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.2 sprout
-
 Pisum sativum
-
5.1.3.5 sprout
-
 Arabidopsis thaliana
-
5.1.3.5 sprout
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.2 1.7
-
 native protein, substrate is UDP-glucose Pisum sativum
5.1.3.2 1.7
-
 purified native enzyme, substrate UDP-Glc Pisum sativum
5.1.3.2 4.6
-
 purified recombinant detagged enzyme, substrate UDP-Glc Pisum sativum
5.1.3.2 4.6
-
 recombinant Pisum protein, substrate is UDP-glucose Pisum sativum
5.1.3.2 6.67
-
 purified native enzyme, substrate UDP-D-Xyl Pisum sativum
5.1.3.2 6.7
-
 purified native enzyme, substrate UDP-L-Ara Pisum sativum
5.1.3.2 16.7
-
 specific activity of recombinant enzyme in cell lysate after expression in Escherichia coli Pisum sativum
5.1.3.2 19.8
-
 native protein, substrate is UDP-galactose Pisum sativum
5.1.3.2 19.8
-
 purified native enzyme, substrate UDP-Gal Pisum sativum
5.1.3.2 21.1
-
 purified recombinant detagged enzyme, substrate UDP-L-Ara Pisum sativum
5.1.3.2 38.9
-
 purified recombinant detagged enzyme, substrate UDP-D-Xyl Pisum sativum
5.1.3.2 38.9
-
 specific activity of recombinant enzyme after purification by a DEAE-sepharose FF column Pisum sativum
5.1.3.2 49
-
 specific activity of recombinant enzyme after purification by a Ni-sepharose colum Pisum sativum
5.1.3.2 53.3
-
 purified recombinant detagged enzyme, substrate UDP-Gal Pisum sativum
5.1.3.2 53.3
-
 recombinant Pisum protein, substrate is UDP-galactose Pisum sativum
5.1.3.5 6.7
-
 native protein, substrate is UDP-arabinose Pisum sativum
5.1.3.5 6.7
-
 native protein, substrate is UDP-xylose Pisum sativum
5.1.3.5 16.7
-
 specific activity of recombinant enzyme in cell lysate after expression in Escherichia coli Pisum sativum
5.1.3.5 21.1
-
 recombinant Pisum protein, substrate is UDP-arabinose Pisum sativum
5.1.3.5 22.4
-
 recombinant Pisum protein, substrate is UDP-xylose Pisum sativum
5.1.3.5 38.9
-
 specific activity of recombinant enzyme after purification by a DEAE-sepharose FF column Pisum sativum
5.1.3.5 49
-
 specific activity of recombinant enzyme after purification by a Ni-sepharose colum Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 additional information the bifunctional UDP-Glc 4-epimerase/UDP-Xyl 4-epimerase in the cytosol is distinct from the UDP-Xyl 4-epimerase in the Golgi apparatus, the two activities of UGE1 occur at the same catalytic site. No activity with UDP-N-acetylglucosamine or UDP-glucuronic acid, UGE1 substrate specificity, overview Pisum sativum ?
-
 ?
5.1.3.2 UDP-alpha-D-glucose
-
 Pisum sativum UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-alpha-D-glucose the apparent equilibrium constant for UDP-Gal formation from UDP-Glc is 0.24 Pisum sativum UDP-alpha-D-galactose
-
 r
5.1.3.2 UDP-D-glucose
-
 Arabidopsis thaliana UDP-D-galactose
-
 r
5.1.3.2 UDP-D-glucose
-
 Pisum sativum UDP-D-galactose
-
 r
5.1.3.2 UDP-D-xylose
-
 Pisum sativum UDP-L-arabinose
-
 r
5.1.3.2 UDP-D-xylose the apparent equilibrium constant for UDP-Ara formation from UDP-Xyl is 0.89 Pisum sativum UDP-L-arabinose
-
 r
5.1.3.2 UDP-galactose
-
 Pisum sativum UDP-glucose
-
 r
5.1.3.2 UDP-galactose the apparent equilibrium constant for UDP-Gal formation from UDP-Glc is 0.24 Pisum sativum UDP-glucose
-
 r
5.1.3.2 UDP-L-arabinose the apparent equilibrium constant for UDP-Ara formation from UDP-Xyl is 0.89 Pisum sativum UDP-D-xylose
-
 r
5.1.3.5 UDP-D-xylose
-
 Arabidopsis thaliana UDP-L-arabinose
-
 r
5.1.3.5 UDP-D-xylose
-
 Pisum sativum UDP-L-arabinose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 AtUGE1
-
 Arabidopsis thaliana
5.1.3.2 AtUGE2
-
 Arabidopsis thaliana
5.1.3.2 AtUGE3
-
 Arabidopsis thaliana
5.1.3.2 AtUGE4
-
 Arabidopsis thaliana
5.1.3.2 AtUGE5
-
 Arabidopsis thaliana
5.1.3.2 More PsUGE1 belongs to plant UGE I family Pisum sativum
5.1.3.2 PsUGE1
-
 Pisum sativum
5.1.3.2 UDP-Glc 4-epimerase
-
 Arabidopsis thaliana
5.1.3.2 UDP-Glc 4-epimerase
-
 Pisum sativum
5.1.3.2 UDP-glucose 4-epimerase
-
 Arabidopsis thaliana
5.1.3.2 UDP-glucose 4-epimerase
-
 Pisum sativum
5.1.3.2 UDP-Xyl 4-epimerase
-
 Pisum sativum
5.1.3.2 UDP-xylose 4-epimerase
-
 Pisum sativum
5.1.3.2 UGE1
-
 Pisum sativum
5.1.3.5 AtUGE1
-
 Arabidopsis thaliana
5.1.3.5 AtUGE2
-
 Arabidopsis thaliana
5.1.3.5 AtUGE3
-
 Arabidopsis thaliana
5.1.3.5 AtUGE4
-
 Arabidopsis thaliana
5.1.3.5 AtUGE5
-
 Arabidopsis thaliana
5.1.3.5 PsUGE1
-
 Pisum sativum
5.1.3.5 UDP-Xyl 4-epimerase
-
 Arabidopsis thaliana
5.1.3.5 UDP-Xyl 4-epimerase
-
 Pisum sativum
5.1.3.5 UDP-xylose 4-epimerase
-
 Arabidopsis thaliana
5.1.3.5 UDP-xylose 4-epimerase
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.2 15
-
 recombinant enzyme Pisum sativum
5.1.3.2 15
-
 maximum activity of recombinant PsUGE1 Pisum sativum
5.1.3.2 30
-
 native enzyme Pisum sativum
5.1.3.2 30
-
 maximum activity of native enzyme Pisum sativum
5.1.3.5 15
-
 maximum activity of recombinant enzyme Pisum sativum
5.1.3.5 25
-
 assay at Arabidopsis thaliana
5.1.3.5 30
-
 maximum activity of native enzyme Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.2 40
-
 purified recombinant enzyme, complete inactivation above Pisum sativum
5.1.3.2 40
-
 recombinant Pisum enzyme loses both activities completely at temperatures higher than 40°C Pisum sativum
5.1.3.5 40
-
 recombinant Pisum enzyme loses both activities completely at temperatures higher than 40°C Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.2 9
-
 UDP-glucose pH 8.6, 25°C Pisum sativum
5.1.3.2 9
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
5.1.3.2 17
-
 UDP-D-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 17
-
 UDP-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 20
-
 UDP-D-galactose recombinant enzyme UGE4 Arabidopsis thaliana
5.1.3.2 23
-
 UDP-L-arabinose pH 8.6, 25°C Pisum sativum
5.1.3.2 27
-
 UDP-D-galactose recombinant enzyme UGE3 Arabidopsis thaliana
5.1.3.2 55
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme UGE2 Arabidopsis thaliana
5.1.3.2 64
-
 UDP-galactose pH 8.6, 25°C Pisum sativum
5.1.3.2 64
-
 UDP-D-galactose recombinant enzyme UGE5 Arabidopsis thaliana
5.1.3.5 0.2
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
5.1.3.5 0.4
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
5.1.3.5 0.5
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
5.1.3.5 13
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3 Arabidopsis thaliana
5.1.3.5 19
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 8.5 9 recombinant enzyme Pisum sativum
5.1.3.2 8.6
-
 assay at Arabidopsis thaliana
5.1.3.2 8.6
-
 assay at Pisum sativum
5.1.3.5 8.6
-
 assay at Arabidopsis thaliana
5.1.3.5 8.6
-
 assay at Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.2 8.5 9 maximum activity of recombinant PsUGE1 Pisum sativum
5.1.3.5 8.5 9 maximum activity of recombinant enzyme Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.2 additional information an NAD+ binding motif is GGXGXXG, not required for activity Pisum sativum

General Information

EC Number General Information Comment Organism
5.1.3.2 physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Arabidopsis thaliana
5.1.3.2 physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Pisum sativum
5.1.3.5 physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Arabidopsis thaliana
5.1.3.5 physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Pisum sativum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.2 28
-
 UDP-glucose pH 8.6, 25°C Pisum sativum
5.1.3.2 103
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.2 115
-
 UDP-D-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 115
-
 UDP-xylose pH 8.6, 25°C Pisum sativum
5.1.3.2 141
-
 UDP-L-arabinose pH 8.6, 25°C Pisum sativum
5.1.3.2 220
-
 UDP-galactose pH 8.6, 25°C Pisum sativum
5.1.3.2 350
-
 UDP-D-galactose recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.2 397
-
 UDP-D-galactose recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.2 435
-
 UDP-D-galactose recombinant enzyme UGE5, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.2 578
-
 UDP-D-galactose recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase Arabidopsis thaliana
5.1.3.5 0.5
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE4, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 1.2
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 3.1
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE5, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 40
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
5.1.3.5 45
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana